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Identification of cytoprotective constituents of the flower buds of Tussilago farfara against glucose oxidase-induced oxidative stress in mouse fibroblast NIH3T3 cells and human keratinocyte HaCaT cells

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Abstract

A new cytoprotective compound, 1-[(4S)-3,4-dihydro-4-hydroxy-2,2-dimethyl-2H-1-benzopyran-6-yl]-ethanone (1) was isolated from the flower buds of Tussilago farfara L. (Compositae), together with eight known compounds, 3,4-dicaffeoyl isoquinic acid (2), trans-cinnamic acid (3), 4-hydroxyacetophenone (4), 4,5-dicaffeoylquinic acid methyl ester (5), 3,5-dicaffeoylquinic acid methyl ester (6), 4-hydroxybenzoic acid (7), isoquercetrin (8), and ligucyperonol (9). Compounds 2–4 were found in this plant for the first time. The isolates 1–9, were tested for their cytoprotective activities against glucose oxidase-induced oxidative stress in mouse fibroblast NIH3T3 cells and human keratinocyte HaCaT cells. Among them, 1 and 3 showed significant cytoprotective activities as determined by MTT assay and lactate dehydrogenase leakage, indicating their possibility as the potent cytoprotective agents. The structure of 1 was determined by spectroscopic data analysis including 1D- and 2D-NMR experiments, and its absolute configuration was elucidated by a circular dichroism.

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Acknowledgments

This research was funded by a Grant (12172MFDS989) from Ministry of Food and Drug Safety in 2014, and was also supported by Global PhD Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2012H1A2A1017710).

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Authors and Affiliations

  1. College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 03760, Korea

    Unwoo Kang, Jiyoung Park, Ah-Reum Han, Tong-Shin Chang, Hyun Ae Woo & Eun Kyoung Seo

  2. College of Pharmacy, Catholic University of Daegu, Gyeongsan, 38430, Korea

    Mi Hee Woo

  3. Department of Korean Medicine, Dongguk University, Gyeongju, 38066, Korea

    Je-Hyun Lee

  4. College of Pharmacy, Seoul National University, Seoul, 08826, Korea

    Sang Kook Lee

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  1. Unwoo Kang
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  2. Jiyoung Park
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  3. Ah-Reum Han
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  4. Mi Hee Woo
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  5. Je-Hyun Lee
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  6. Sang Kook Lee
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  7. Tong-Shin Chang
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  8. Hyun Ae Woo
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Correspondence to Hyun Ae Woo or Eun Kyoung Seo.

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Kang, U., Park, J., Han, AR. et al. Identification of cytoprotective constituents of the flower buds of Tussilago farfara against glucose oxidase-induced oxidative stress in mouse fibroblast NIH3T3 cells and human keratinocyte HaCaT cells. Arch. Pharm. Res. 39, 474–480 (2016). https://doi.org/10.1007/s12272-016-0730-z

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  • DOI: https://doi.org/10.1007/s12272-016-0730-z

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