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Four pestalpolyols from a mycoparasite Pestalotipsis sp. PG52


A mycoparasite PG52 isolated from aeciospore piles of Aecidium pourthiaea was identified as Pestalotipsis sp. The chemical constituents of Pestalotipsis sp. PG52 were investigated. Four novel polyketides, named pestalpolyols E–H (14), were isolated from solid fermentations of Pestalotiopsis sp. PG52. Their structures were elucidated by interpretation of extensive spectroscopic methods, including 1D and 2D NMR, HR-MS experiments; and the absolute configuration of 1 was confirmed by single-crystal X-ray diffraction analysis using the anomalous scattering of Cu Kα radiation. Compounds 24 showed cytotoxic activities against human tumor cell lines.

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  1. Cimino G, Sodano G, Spinella A (1985) Aglajne-1, a polypropionate metabolite from the opisthobranch mollusk Aglaja depicta: Determination of carbon-carbon connectivity via long-range 1H-13C couplings. Tetrahedron Lett 26:3389–3392

    CAS  Article  Google Scholar 

  2. Flack HD, Bernardinelli G (2008) The use of X-ray crystallography to determine absolute configuration. Chirality 20(5):681–690

    CAS  Article  PubMed  Google Scholar 

  3. Hooft RW, Straver LH, Spek AL (2008) Determination of absolute structure using Bayesian statistics on Bijvoet differences. J Appl Crystallogr 41:96–103

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  4. Hopkins KE, McQuilken MP (2000) Characteristics of Pestalotiopsis associated with hardy ornamental plants in the UK. Eur J Plant Pathol 106:77–85

    Article  Google Scholar 

  5. Kasai Y, Komatsu K, Shigemori H, Tsuda M, Mikami Y, Kobayashi J, Cladionol A (2005) A polyketide glycoside from marine-derived fungus Gliocladium species. J Nat Prod 68:777–779

    CAS  Article  PubMed  Google Scholar 

  6. Keith LM, Velasquez ME, Zee FT (2006) Identification and characterization of Pestalotiopsis spp. causing scab disease of guava, Psidium guajava, in Hawaii. Plant Dis 90:16–23

    CAS  Article  Google Scholar 

  7. Lee JC, Strobel GA, Lobkovsky E, Clardy J (1996) Torreyanic acid: a selectively cytotoxic quinone dimer from the endophytic fungus Pestalotiopsis microspora. J Org Chem 61:3232–3233

    CAS  Article  Google Scholar 

  8. Li EW, Jiang LH, Guo LD, Zhang H, Che YS (2008) Pestalachlorides A-C, antifungal metabolites from the plant endophytic fungus Pestalotiopsis adusta. Bioorg Med Chem 16:7894–7899

    CAS  Article  PubMed  Google Scholar 

  9. Liu L, Li Y, Liu SC, Zheng ZH, Chen XL, Zhang H, Guo LD, Che YS (2009) Chloropestolide A, an antitumor metabolite with an unprecedented spiroketal skeleton from Pestalotiopsis fici. Org Lett 11:2836–2839

    CAS  Article  PubMed  Google Scholar 

  10. Maharachchikumbura SS, Guo LD, Chukeatirote E, Bahkali AH, Hyde KD (2011) Pestalotiopsis—morphology, phylogeny, biochemistry and diversity. Fungal Divers 50:167–187

    Article  Google Scholar 

  11. Strobel G, Daisy B, Castillo U, Harper J (2004) Natural products from endophytic microorganisms. J Nat Prod 67:257–268

    CAS  Article  PubMed  Google Scholar 

  12. Su J, Zhao P, Kong L, Li X, Yan J, Zeng Y, Li Y (2013) Trichothecin induces cell death in NF-κB constitutively activated human cancer cells via inhibition of IKKβ phosphorylation. PLoS One 8(8):e71333

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  13. Taylor JE, Crous PW, Palm ME (2001) Foliar and stem fungal pathogens of Proteaceae in Hawaii. Mycotaxon 78:449–490

    Google Scholar 

  14. Xu J, Ebada SS, Proksch P (2010) Pestalotiopsis a highly creative genus: chemistry and bioactivity of secondary metabolites. Fungal Divers 44:15–31

    Article  Google Scholar 

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This work was supported by the National Basic Research Program of China (973 Program, 2013CB127505), the NSFC (31170061) and the Applied Basic Research Foundation of Yunnan Province (2013FA018). We acknowledge the Department of Instrumental Analysis of Kunming Institute of Botany for measuring the optical rotations, UV, NMR, X-ray and mass spectra.

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Correspondence to Pei-Ji Zhao.

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Jin Xie and Jing Li have contributed equally to this work.

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Xie, J., Li, J., Yang, YH. et al. Four pestalpolyols from a mycoparasite Pestalotipsis sp. PG52. Arch. Pharm. Res. (2015).

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  • Pestalotiopsis sp. PG52
  • Pestalpolyol
  • Polyketide
  • Cytotoxic activity