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A new naphthalene glycoside from Chimaphila umbellata inhibits the RANKL-stimulated osteoclast differentiation

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Abstract

A new naphthalene glycoside was isolated from the leaves and stems of Chimaphila umbellata Barton. Its chemical structure was elucidated to be 2,7-dimethyl-1,4-dihydroxynaphthalene-1-O-β-d-glucopyranoside (DMDHNG), based on spectroscopic evidence. DMDHNG significantly inhibited the receptor activator of nuclear factor-κB ligand (RANKL)-induced tartrate-resistant acid phosphatase (TRAP) activity and the formation of multinucleated osteoclasts in a dose-dependent manner. In addition, the new glycoside inhibited the RANKL-induced mRNA expression of osteoclast-associated genes that encode TRAP, cathepsin K, and another transcription factor—nuclear factor of activated T-cells c1. We believe that the inhibitory effects of DMDHNG on the osteoclast differentiation may be exploited for a therapeutic benefit.

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Acknowledgments

This work was supported by the Bio-Synergy Research Project (NRF-2012M3A9C4048796) of the Ministry of Science, ICT and Future Planning through the National Research Foundation. The authors wish to acknowledge the financial support of Gachon University Research Fund.

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The authors declare no conflict of interest.

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    Authors and Affiliations

    1. College of Pharmacy, Seoul National University, Seoul, 151-742, Republic of Korea

      Byong-kyu Shin & Jeong Hill Park

    2. College of Korean Medicine, Gachon University, Seongnam, 461-701, Republic of Korea

      Jinhee Kim, Ki Sung Kang & Gwi Seo Hwang

    3. College of Pharmacy, Yanbian University, Yangji, Jilin, 133002, China

      Hui-Shan Piao

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    1. Byong-kyu Shin
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    2. Jinhee Kim
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    3. Ki Sung Kang
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    Correspondence to Jeong Hill Park or Gwi Seo Hwang.

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    Byong-kyu Shin and Jinhee Kim contributed equally to this work.

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    Shin, Bk., Kim, J., Kang, K.S. et al. A new naphthalene glycoside from Chimaphila umbellata inhibits the RANKL-stimulated osteoclast differentiation. Arch. Pharm. Res. 38, 2059–2065 (2015). https://doi.org/10.1007/s12272-015-0598-3

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