Abstract
To establish a standard of quality control for Perillae Folium (Lamiaceae Family), four standard compounds including rosmarinic acid, elemicin, perillaldehyde, and dillapiole were evaluated by high-performance liquid chromatography (HPLC)/photodiode array (PDA). The four standards were analyzed with a Phenomenex Kinetex C18 (250 × 4.6 mm, 5 μm) column by gradient elution using 0.1 % formic acid in water and methanol as the mobile phase. The standards were quantified by HPLC/PDA from Perillae Folium, which included the leaf and twig of Perilla frutescens L. Britton var. acuta (Thunb.) Kudo or Perilla frutescens Britton var. crispa Decne. The method was successfully used in the analysis of Perillae Folium, and the linearity, recovery, precision, accuracy, stability, and robustness were satisfactory according to the validation results. In Perillae Folium samples, the average contents (wt%) of rosmarinic acid, elemicin, perillaldehyde, and dillapiole were 0.540, 0.059, 0.050, and 0.056 %, respectively. The results indicate that the established HPLC/PDA method is suitable for the quantitation and quality evaluation of Perillae Folium.
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Acknowledgments
This work was supported by a Grant from the National Center for Standardization of Herbal Medicine funded by the Ministry of Food and Drug Safety, Republic of Korea (12172MFDS890), and National Research Foundation of Korea (BK21 Plus; 22A20130000073).
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The authors have no conflicts of interest that are directly relevant to the content of this study.
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Zhao, B.T., Lee, K.R., Lee, J.H. et al. Quality evaluation of Perillae Folium by HPLC/PDA. Arch. Pharm. Res. 38, 1521–1529 (2015). https://doi.org/10.1007/s12272-015-0582-y
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DOI: https://doi.org/10.1007/s12272-015-0582-y