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Anti-inflammatory effects of Dendrobium nobile derived phenanthrenes in LPS-stimulated murine macrophages

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Abstract

Dendrobium nobile belongs to the Orchidaceae family and is one of the medicinal herbs used in traditional Chinese medicine as a therapeutic agent for gastrointestinal and cardiovascular diseases. In this study, we separated three phenanthrenes (ephemeranthol A (EA), 1,5,7-trimethoxyphenanthren-2-ol (TP), dehydroorchinol (DO)) from D. nobile, and compared their anti-inflammatory activities. TP is a new phenanthrene compound and its structure was determined from 1H, 13C NMR and HR-ESI-MS data. To analyze the anti-inflammatory activities of the phenanthrenes, Raw 264.7 cells were used, since they are immature-macrophages and easily matured by LPS stimulation. EA and DO showed anti-inflammatory activities in the activated Raw 264.7 cells. That is, we showed that EA is a potent inhibitor of the production of nitric oxide and pro-inflammatory cytokines. The inhibitory activities of phenanthrenes were found to be caused by blockage of NF-κB activation and the phosphorylation of MAP kinases in the macrophages. These results are expected to serve as a guide for future studies on the ability of phenanthrenes to inhibit acute and chronic inflammatory diseases.

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Acknowledgments

This work was supported by the research Grant of the Chungbuk National University in 2013.

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Authors and Affiliations

  1. Department of Biology Education, College of Education, Chungbuk National University, Cheongju, Chungcheongbuk-Do, 361-763, Republic of Korea

    Jeong Hwa Kim, Su-Yeon Oh & Jae Kwon Lee

  2. College of Pharmacy, Chungbuk National University, Cheongju, Chungcheongbuk-Do, 361-763, Republic of Korea

    Sang-Bae Han

  3. Functional Food Center, KIST Gangneung Institute, Techno Valley, Gangneung, Gangwon-Do, 210-340, Republic of Korea

    Golam Mezbah Uddin & Chul Young Kim

  4. College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi-Do, 426-791, Republic of Korea

    Chul Young Kim

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  1. Jeong Hwa Kim
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Correspondence to Chul Young Kim or Jae Kwon Lee.

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Kim, J.H., Oh, SY., Han, SB. et al. Anti-inflammatory effects of Dendrobium nobile derived phenanthrenes in LPS-stimulated murine macrophages. Arch. Pharm. Res. 38, 1117–1126 (2015). https://doi.org/10.1007/s12272-014-0511-5

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  • DOI: https://doi.org/10.1007/s12272-014-0511-5

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