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Inhibitory effects of SKI3246, the rhizome extract of Atractylodes japonica, on visceral hypersensitivity in experimental irritable bowel syndrome rat models

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Abstract

We evaluated the effect of SKI3246, the 50 % ethanol extract of the rhizome of Atractylodes japonica, on visceral hypersensitivity, which is a major characteristic feature of IBS. We used various rat models of visceral hypersensitivity to assess the visceral pain responses to colorectal distension (CRD) in comparison with conventional IBS treatments. Oral administration of SKI3246 dose-dependently and significantly attenuated the abdominal withdrawal reflex (AWR) score in a model of acetic acid-induced visceral hypersensitivity. We also found that it reduced the number of abdominal contractions in response to CRD in a model of 2,4,6-trinitrobenzenesulfonic acid-induced visceral hypersensitivity, which was comparable to ramosetron or alosetron. Furthermore, treatment with SKI3246 also increased the pain threshold and abolished the elevated AWR scores to CRD in a rat model of neonatal maternal separation. We presumed that the modulation of the NK2 receptor is involved in the inhibitory activity of SKI3246 on the basis that it significantly inhibited the contraction of the distal colonic muscle induced by neurokinin A, the NK2 receptor agonist. The present results indicate that SKI3246 has the potential to be an effective therapeutic agent for IBS, especially insofar as it can relieve visceral hypersensitivity.

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Acknowledgments

This study was supported by a grant of the Korea Health technology R&D Project, Ministry of Health & Welfare, Republic of Korea. (Grant Number: A120767).

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Authors and Affiliations

  1. Life Science R&D Center, SK Chemicals, Seongnam, 463-400, Gyeonggi-do, Republic of Korea

    Hyun Joo Son, Yang Hae Park, Hyo Jin Jeon, Minseok Kang, Keun Ho Ryu, Sung Soo Pyo & Won Suk Sun

  2. Department of Pharmacy, CHA University, 120 Haeryong-ro, Pocheon-shi, Gyeonggi-do, Republic of Korea

    Kiwon Jung

  3. Department of Neuro-Gastroenterology and Nutrition Toxalim, UMR 131 INRA/EI-Purpan, Toulouse, France

    Helene Eutamene & Lionel Bueno

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  1. Hyun Joo Son
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Correspondence to Won Suk Sun.

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Son, H.J., Jung, K., Park, Y.H. et al. Inhibitory effects of SKI3246, the rhizome extract of Atractylodes japonica, on visceral hypersensitivity in experimental irritable bowel syndrome rat models. Arch. Pharm. Res. 38, 642–649 (2015). https://doi.org/10.1007/s12272-014-0454-x

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