Alloxan-induced diabetic thermal hyperalgesia, prophylaxis and phytotherapeutic effects of Rheum ribes L. in mouse model
- 380 Downloads
Rheum ribes L., known as Syrian rhubarb, is used in traditional Lebanese folk medicine for the treatment of diabetes. The present study aims to investigate the activities of R. ribes aqueous extract for glucose homeostasis, in vivo antioxidant and diabetic neuropathy protection in mice. The acute and the subacute effects of various doses of R. ribes on blood glucose and in vivo antioxidant activity utilizing serum catalase level (CAT) were studied in alloxan-diabetic mice. The high doses significantly lowered glucose level and increased serum CAT in alloxan-diabetic mice. Pretreatment with the extract prior to alloxination, protected the mice from acquiring diabetes and diabetic neuropathy. Treatment with the extract for 8 weeks alleviated hyperalgesia in diabetic mice. Our findings provide clinicians with promising drugs intended for the management of the symptoms of diabetic complications. The protective activity of R. ribes against acquiring diabetes and diabetic neuropathy might pave the way for preparing a prophylactic treatment for diabetes risk groups.
KeywordsRheum ribes L. Diabetes Diabetic neuropathy Alloxan Pain In-vivo antioxidant
Rutin rich fraction
Lethality towards 50 % of a population
- R. ribes
Reactive oxygen species
Authors would like to thank the Junior Research Team, Department of Pharmaceutical Sciences, Faculty of Pharmacy, BAU for helping in the methodology preparation.
Conflict of interest
The authors declare that there is no conflict of interest.
- Harati, Y., C. Gooch, M. Swenson, S.V. Edelman, D. Greene, P. Raskin, P. Donofrio, D. Cornblath, W.H. Olson, and M. Kamin. 2000. Maintenance of the long-term effectiveness of tramadol in treatment of the pain of diabetic neuropathy. Journal of Diabetes and Its Complications 14: 65–70.PubMedCrossRefGoogle Scholar
- Oktay, M., A. Yildirim, V. Bilaloglu, and I. Gulcin. 2007. Antioxidant activity of different parts of isgin (Rheum ribes L.). Asian Journal of Chemistry 19: 3047–3055.Google Scholar
- Özbek, H., E. Ceylan, M. Kara, F. Özgökçe, and M. Koyuncu. 2004a. Hypoglycemic effect of Rheum ribes roots in alloxan induced diabetic and normal mice. Scandinavian Journal of Laboratory Animal Science 31: 113–115.Google Scholar
- Özbek, H., E. Ceylan, M. Kara, F. Özgökçe, and M. Koyuncu. 2004b. Hypoglycemic effect of Rheum ribes roots in alloxan induced diabetic and normal mice. Scandinavian Journal of Laboratory Animal Science 31: 113–115.Google Scholar
- Punitha, I.S., K. Rajendran, and A. Shirwaikar. 2005. Alcoholic stem extract of Coscinium fenestratum regulates carbohydrate metabolism and improves antioxidant status in streptozotocin-nicotinamide induced diabetic rats. Evidence-Based Complementary and Alternative Medicine 2: 375–381.PubMedCentralPubMedCrossRefGoogle Scholar
- Raafat, K., R. Boukhary, M. Aboul-Ela, and A. El-Lakany. 2013a. Endogenous Lebanese plants treating diabetes and related complications. Natural Products Chemistry & Research 1: 112–120.Google Scholar
- Raafat, K.M., H. Jassar, M. Aboul-Ela, and A. El-Lakany. 2013b. Protective effects of Origanum majorana L. against neurodegeneration: Fingerprinting, isolation and in vivo glycine receptors behavioral model. Inernational Journal of Phytomedicine 5: 46–57.Google Scholar
- Tesfaye, S., A.J. Boulton, P.J. Dyck, R. Freeman, M. Horowitz, P. Kempler, G. Lauria, R.A. Malik, V. Spallone, A. Vinik, L. Bernardi, and P. Valensi. 2010. Diabetic neuropathies: Update on definitions, diagnostic criteria, estimation of severity, and treatments. Diabetes Care 33: 2285–2293.PubMedCentralPubMedCrossRefGoogle Scholar
- Tosun, F., and Ç.A. Kizilay. 2003. Anthraquinone and flavonoids from Rheum ribes. Journal of Faculty of Pharmacy of Ankara 32: 31–35Google Scholar
- Ueno, Y., Koike, H., Annoh, S., and Nishio, S. 1996. Effects of beraprost sodium, a prostacyclin analogue, on tail flick response in two models of diabetic-neuropathy in rats and its mechanism. Life Science 59: PL105–PL110.Google Scholar
- Vinik, A.I., V. Bril, P. Kempler, W.J. Litchy, S. Tesfaye, K.L. Price, and E.J. Bastyr III. 2005. Treatment of symptomatic diabetic peripheral neuropathy with the protein kinase C beta-inhibitor ruboxistaurin mesylate during a 1-year, randomized, placebo-controlled, double-blind clinical trial. Clinical Therapeutics 27: 1164–1180.PubMedCrossRefGoogle Scholar