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Nonviral delivery for reprogramming to pluripotency and differentiation

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Abstract

Nonviral delivery is a promising strategy for cellular reprogramming to produce desired cell types from undifferentiated stem cells or terminally differentiated somatic cells. Nonviral delivery of genes (DNA, RNA), proteins, or peptides has the potential to reprogram somatic cells to pluripotent stem cells or other lineage cells, and to promote the differentiation of stem cells to specific lineages. Various delivery carriers (cationic polymers, lipids, scaffolds, transposons, cell-penetrating peptides), cargos (episomal plasmids, minicircle DNA, small interfering RNAs, microRNAs, proteins, peptides), and method (electroporation) have been reported. In this article, we review recent advances in nonviral delivery approaches for reprogramming cells to pluripotency or lineage specification.

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Acknowledgments

This study was supported by a Grant (2010-0020409) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP), Republic of Korea. This work was also supported in part by a Grant (10035159) funded by the Ministry of Knowledge Economy, Republic of Korea. Jin Kim was supported by the NRF-2013-Global Ph.D. fellowship program.

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  1. Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-749, Republic of Korea

    Hyun-Ji Park, Jisoo Shin, Jin Kim & Seung-Woo Cho

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  1. Hyun-Ji Park
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  2. Jisoo Shin
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  3. Jin Kim
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Correspondence to Seung-Woo Cho.

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Hyun-Ji Park and Jisoo Shin have contributed equally to this work.

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Park, HJ., Shin, J., Kim, J. et al. Nonviral delivery for reprogramming to pluripotency and differentiation. Arch. Pharm. Res. 37, 107–119 (2014). https://doi.org/10.1007/s12272-013-0287-z

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  • DOI: https://doi.org/10.1007/s12272-013-0287-z

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