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A simultaneous analysis method for Caihu-Dayuan-Yin using reversed-phase high-performance liquid chromatography coupled with pulsed amperometric detection

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Abstract

A simultaneous analysis method was developed for Caihu-Dayuan-Yin decoction containing Bupleuri Radix, Glycyrrhizae Radix, and Ponciri fructus by reversed-phase (RP) HPLC coupled with pulsed amperometric detection (PAD). The analytes were separated in a RP column with 0.01 % phosphoric acid–acetonitrile gradient elution. The limits of detection (S/N = 3) and of quantification (S/N = 10) ranged 0.1–3.0, and 0.3–10 ng, respectively. The intra- and interday precisions (RSDs) were ≤15.15 % and the average recoveries were 94.6–98.3 %. Our RP-HPLC–PAD method showed 1.7–100 times higher sensitivity than the RP-HPLC–UV method.

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Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012-0003628).

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Authors and Affiliations

  1. Department of Preventive and Social Dentistry, Graduate School, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea

    Ha-Jeong Kwon & Yong-Duk Park

  2. Department of Dental Laboratory Science & Engineering, College of Health Science, Korea University, Seoul, Republic of Korea

    Ji-Hwan Kim

  3. Department of Oriental Pharmaceutical Sciences, Kyung Hee East–West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea

    Seon-Pyo Hong

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  1. Ha-Jeong Kwon
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  2. Ji-Hwan Kim
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  3. Yong-Duk Park
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  4. Seon-Pyo Hong
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Corresponding authors

Correspondence to Yong-Duk Park or Seon-Pyo Hong.

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Yong-Duk Park and Seon-Pyo Hong contributed equally to this study.

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Kwon, HJ., Kim, JH., Park, YD. et al. A simultaneous analysis method for Caihu-Dayuan-Yin using reversed-phase high-performance liquid chromatography coupled with pulsed amperometric detection. Arch. Pharm. Res. 36, 579–585 (2013). https://doi.org/10.1007/s12272-013-0040-7

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