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Effects of the rhizomes of Atractylodes japonica and atractylenolide I on allergic response and experimental atopic dermatitis

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Abstract

Although some anti-allergic activities of the rhizome of Atractylodes japonica have been previously reported, the active principle(s) for anti-allergic action is not fully elucidated and the effect of this plant material on atopic dermatitis (AD) is not known. In this study, the 70% ethanol extract of the rhizome of A. japonica was found to significantly inhibit 5-lipoxygenase (5-LOX)-catalyzed leukotrienes (LT) production from rat basophilic leukemia (RBL)-1 cells. From the extract of A. japonica, three major sesquiterpene derivatives including atractylenolide I, atractylenolide III and eudesma-4,7-dien-8-one were successfully isolated. Among these compounds, only atractylenolide I was shown to strongly inhibit 5-LOX from RBL-1 cells (IC50 = 18.6 μM). To evaluate the effects of experimental AD, the ethanol extract of A. japonica (200 mg/day) was administered orally to hapten-treated NC/Nga mice which is an animal model of AD. It was firstly found that the extract significantly inhibited AD-like symptoms in mice, as judged by severity score and scratching behavior. Taken together, it is concluded that A. japonica possesses the inhibitory activity on 5-LOX and an animal model of AD, and atractylenolide I may contribute, at least in part, to these anti-allergic actions of A. japonica.

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Authors and Affiliations

  1. College of Pharmacy, Kangwon National University, Chunchon, 200-701, Korea

    Hyun Lim, Je Hyeong Lee & Hyun Pyo Kim

  2. College of Pharmacy, Seoul National University, Seoul, 110-460, Korea

    Jinwoong Kim & Yeong Shik Kim

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  1. Hyun Lim
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  2. Je Hyeong Lee
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Correspondence to Hyun Pyo Kim.

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Lim, H., Lee, J.H., Kim, J. et al. Effects of the rhizomes of Atractylodes japonica and atractylenolide I on allergic response and experimental atopic dermatitis. Arch. Pharm. Res. 35, 2007–2012 (2012). https://doi.org/10.1007/s12272-012-1118-3

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  • DOI: https://doi.org/10.1007/s12272-012-1118-3

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