Skip to main content

Qualitative and quantitative simultaneous determination of six marker compounds in soshiho-tang by HPLC-DAD-ESI-MS

Abstract

Soshiho-tang, one of the herbal prescriptions, has been used in treatment of chronic liver disease. In this study, the efficient high-performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI-MS) method was developed and validated for simultaneous determination of six marker compounds, namely homogentisic acid, baicalin, glycyrrhizin, saikosaponin A, 6-gingerol and ginsenoside Rg3 in Soshiho-tang. The analysis of six marker compounds was carried out using a C18 column (SHISEIDO S-5 μm, 4.6 mm I.D. × 250 mm) and gradient elution with water − 0.1% trifluoroacetic acid and acetonitrile. The analytical method was validated for linearity, precision and accuracy. Calibration curve for six marker compounds showed good linear regression (r2 > 0.9996). The limits of detection (LOD) and limits of quantification (LOQ) for analytes were ranged from 0.04 to 0.16 μg/mL and 0.12 to 0.47 μg/mL, respectively. The relative standard deviations (RSD%) of the precision test, intra- and inter-day, were less than 0.32% and 0.10%, respectively. All recoveries of the six marker compounds were ranged from 100.05% to 102.25% with RSD less than 0.63%. These results have shown that this developed method was efficient for the quality evaluation of Soshiho-tang.

References

  1. Chen, H. J., Li X., Chen, J. W., Guo, S., and Cai, B. C., Simultaneous determination of eleven bioactive compounds in Saururus chinensis from different harvesting seasons by HPLC-DAD. J. Pharm. Biomed. Anal., 51, 1142–1146 (2010).

    PubMed  Article  CAS  Google Scholar 

  2. Ganzera, M., Recent advancements and applications in the analysis of traditional Chinese medicines. Planta Med., 75, 776–783 (2009).

    PubMed  Article  CAS  Google Scholar 

  3. Han, Z., Liu, X., Ren, Y., Luan, L., and Wu, Y., A rapid method with ultra-high-performance liquid chromatography-tandem mass spectrometry for simultaneous determination of five type B trichothecenes in traditional Chinese medicines. J. Sep. Sci., 33, 1923–1932 (2010).

    PubMed  Article  CAS  Google Scholar 

  4. He, D., Shan, Y, Wuc, Y., Liu, G., Chen, B., and Yao, S., Simultaneous determination of flavanones, hydroxycinnamic acids and alkaloids in citrus fruits by HPLC-DAD°©ESI/MS. Food Chem., 127, 880–885 (2011).

    Article  CAS  Google Scholar 

  5. Hirayama, C., Okumura, M., Tanikawa, K., Yano, M., Mizuta, M., and Ogawa, N., A multicenter randomized controlled clinical trial of Shosaiko-to in chronic active hepatitis. Gastroenterol. Jpn., 24, 715–719 (1989). ICH., Guidance for Industry, Q2B Validation of Analytical Procedures: Methodology. (1996).

    PubMed  CAS  Google Scholar 

  6. Liang, Y. Z., Xie, P., and Chan, K., Quality control of herbal medicines. J. Chromatogr. B, 812, 53–70 (2004).

    CAS  Google Scholar 

  7. Liu, Q., Zhao, J., Yan, L., Yi, J., and Song, J., Simultaneous determination of five active constitutents in Soshiho-tang by HPLC. China J. Chin. Mater. Med., 35, 708–710 (2010).

    CAS  Google Scholar 

  8. Lu, A. P., Jia, H. W., Xiao, C., and Lu, Q. P., Theory of traditional Chinese medicine and therapeutic method of diseases. World J. Gastroenterol., 10, 1854–1856 (2004).

    PubMed  Google Scholar 

  9. Normile, D., Asian medicine. The new face of traditional Chinese medicine. Science, 299, 188–190 (2003).

    PubMed  Article  CAS  Google Scholar 

  10. Oka, H., Yamamoto, S., Kuroki, T., Harihara, S., Marumo, T., Kim, S. R., Monna, T., Kobayashi, K., and Tango, T., Prospective study of chemoprevention of hepatocellular carcinoma with sho-saiko-to (TJ-9). Cancer, 76, 743–749 (1995).

    PubMed  Article  CAS  Google Scholar 

  11. Tajiri, H., Kozaiwa, K., Ozaki, Y., Miki, K., Shimuzu, K., and Okada, S., Effect of sho-saiko-to(xiao-chai-hu-tang) on HBeAg clearance in children with chronic hepatitis B virus infection and with sustained liver disease. Am. J. Chin. Med., 19, 121–129 (1991).

    PubMed  Article  CAS  Google Scholar 

  12. Tyler, V. E., Phytomedicines: back to the future. J. Nat. Prod., 62, 1589–1592 (1999).

    PubMed  Article  CAS  Google Scholar 

  13. Yan, Z., Yang, X., Wu, J., Su, H., Chen, C., and Chen, Y., Qualitative and quantitative analysis of chemical constituents in traditional Chinese medicinal formula Tong-Xie-Yao-Fang by high-performance liquid chromatography/diode array detection/electrospray ionization tandem mass spectrometry. Anal. Chim. Acta, 691, 110–118 (2011)

    PubMed  Article  CAS  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Choong Je Ma.

Additional information

These authors contributed equally to this work.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Yang, H.J., Ma, J.Y., Weon, J.B. et al. Qualitative and quantitative simultaneous determination of six marker compounds in soshiho-tang by HPLC-DAD-ESI-MS. Arch. Pharm. Res. 35, 1785–1791 (2012). https://doi.org/10.1007/s12272-012-1011-0

Download citation

Key words

  • Simultaneous determination
  • Method validation
  • Soshiho-tang
  • HPLC-DAD-ESI-MS