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Inhibition of DNA topoisomerases I and II of compounds from Reynoutria japonica

Archives of Pharmacal Research volume 35, pages 1583–1589 (2012)Cite this article

Abstract

Three anthraquinones (1, 2 and 4), three stilbenes (5, 6 and 7) and 3,5-dihydroxybenzyl alcohol (3) were isolated from Reynoutria japonica. Their structures were identified as emodin (1), emodin-8-O-β-d-glucoside (2), 3,5-dihydroxybenzyl alcohol (3), citreorosein (4), cis-resveratrol (5), trans-resveratrol (6) and trans-resveratrol-5-O-β-d-glucopyranoside (7) by comparing their physicochemical and spectral data with published data. Compound 3 was isolated for the first time from the Polygonaceae family. Among the purified compounds, 3 showed more potent inhibitory activity against topoisomerase I (IC50: 4 μM) than camptothecin, as the positive control (IC50: 18 μM). Compounds 3, 4, 5, 6 and 7 showed stronger inhibitory activities toward DNA topoisomerase II (IC50: 0.54, 14, 15, 0.77 and 3 μM, respectively) than the positive control, etoposide (IC50: 44 μM). Compounds 1 and 4 displayed weak cytotoxicities against human lung cancer (A549), ovarian cancer (SK-OV-3), human liver hepatoblastoma (HepG2) and colon adenocarcinoma (HT-29) cell lines.

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Affiliations

  1. College of Pharmacy, Yeungnam University, Gyongsan, 712-749, Korea

    Kyoung Hwangbo, Ming Shan Zheng, Young-Jin Kim, Yurngdong Jahng, Hyun-Wook Chang & Jong-Keun Son

  2. College of Pharmacy, Yanbian University, Yanji, 133000, China

    Ming Shan Zheng

  3. School of Biotechnology, Yeungnam University, Gyongsan, 712-749, Korea

    Jong-Yeop Im & Chong-Soon Lee

  4. College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, Korea

    Mi-Hee Woo

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  1. Kyoung Hwangbo
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  2. Ming Shan Zheng
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  9. Jong-Keun Son
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Correspondence to Jong-Keun Son.

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Thess authors contributed equally to this work.

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Hwangbo, K., Zheng, M.S., Kim, YJ. et al. Inhibition of DNA topoisomerases I and II of compounds from Reynoutria japonica . Arch. Pharm. Res. 35, 1583–1589 (2012). https://doi.org/10.1007/s12272-012-0909-x

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  • DOI: https://doi.org/10.1007/s12272-012-0909-x

Key words

  • Reynoutria japonica
  • Polygonaceae
  • Topoisomerase
  • Cytotoxicity