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Anti-inflammatory activity of 6-hydroxy-2,7-dimethoxy-1,4-henanthraquinone from tuberous roots of Yam (Dioscorea batatas) through inhibition of prostaglandin D2 and leukotriene C4 production in mouse bone marrow-derived mast cells

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Abstract

6-Hydroxy-2,7-dimethoxy-1,4-phenanthraquinone (PAQ) isolated from the tuberous roots of Yam (Dioscorea batatas) inhibited cyclooxygenase-2 (COX-2) and cyclooxygenase-1 (COX-1) dependent prostaglandin D2 (PGD2) generation in mouse bone marrow-derived mast cells in a concentration-dependent manner with IC50 values of 0.08 μM and 0.27 μM, respectively. In the Western blotting with specific anti-COX-2 antibodies, the decrease of the quantity of PGD2 was accompanied by a decrease in the COX-2 protein level. But PAQ did not affect COX-1 protein level. In addition, this compound inhibited 5-lipoxygenase (5-LOX) dependent production of leukotriene C4 in a dose-dependent manner, with an IC50 of 0.032 μM. These results demonstrate that PAQ has a dual COX-2/5-LOX inhibitory activity. This compound also inhibited the degranulation reaction in a dose-dependent manner with an IC50 of 2.7 μM. Thus, these results suggest that PAQ may be useful in regulating mast cell-mediated inflammatory diseases.

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Authors and Affiliations

  1. College of Pharmacy, Yeungnam University, Gyeongsan, 712-749, Korea

    Meihua Jin, Yue Lu, Ju Hye Yang & Hyeun Wook Chang

  2. School of Pharmaceutical Sciences, Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070, China

    Meihua Jin

  3. Namyang Inc., Seoul, 133-120, Korea

    Tae Hyung Jo

  4. College of Pharmacy, Korea University, Jochiwon, 339-700, Korea

    Young In Park

  5. College of Pharmacy, Chungbuk National University, Cheongju, 361-763, Korea

    Chong-Kil Lee

  6. Institute for Natural Products Research, Gyeongsangbuk-do Agricultural Research & Extension Services, Uiseong, Gyeongbuk, 760-803, Korea

    Sang-Jo Park

  7. Department of Food Science and Nutrition, Andong National University, Andong, 760-749, Korea

    Kun Ho Son

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  1. Meihua Jin
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Correspondence to Kun Ho Son or Hyeun Wook Chang.

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Jin, M., Lu, Y., Yang, J.H. et al. Anti-inflammatory activity of 6-hydroxy-2,7-dimethoxy-1,4-henanthraquinone from tuberous roots of Yam (Dioscorea batatas) through inhibition of prostaglandin D2 and leukotriene C4 production in mouse bone marrow-derived mast cells. Arch. Pharm. Res. 34, 1495–1501 (2011). https://doi.org/10.1007/s12272-011-0911-8

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  • DOI: https://doi.org/10.1007/s12272-011-0911-8

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