Abstract
Acanthoic acid (AA) is a pimaradiene diterpene isolated from Acanthopanax koreanum. We examined the effect of AA in dextran sulfate sodium (DSS)-induced colitis. AA (100 mg/kg or 300 mg/kg) was administered p.o. daily for 7 days. AA significantly inhibited Disease Activity Index, histological score, and myeloperoxidase activity. Furthermore, AA markedly suppressed the protein expression of TNF-α, COX-2, NF-κB and chymase as well as the mRNA expression of TNF-α and COX-2. These results suggest that AA exerts beneficial effects in experimental colitis, and therefore we propose that this compound may have therapeutic implications for ulcerative colitis.
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References
Fiocchi, C., Inflammatory bowel disease: etiology and pathogenesis. Gastroenterology, 115, 182–205 (1998).
Hendrickson, B. A., Gokhale, R., and Cho, J. H., Clinical aspects and pathophysiology of inflammatory bowel disease. Clin. Microbiol. Rev., 15, 79–94 (2002).
Herfarth, H., Brand, K., Rath, H. C., Rogler, G., Scholmerich, J., and Falk, W., Nuclear factor-kappa B activity and intestinal inflammation in dextran sulphate sodium (DSS)-induced colitis in mice is suppressed by gliotoxin. Clin. Exp. Immunol., 120, 59–65 (2000).
Hirata, I., Murano, M., Nitta, M., Sasaki, S., Toshina, K., Maemura, K., and Katsu, K., Estimation of mucosal inflammatory mediators in rat DSS-induced colitis. Possible role of PGE(2) in protection against mucosal damage. Digestion, 63, 73–80 (2001).
Hyams, J. S., Inflammatory bowel disease. Pediatr. Rev., 21, 291–295 (2000).
Kang, H. S., Kim, Y. H., Lee, C. S., Lee, J. J., Choi, I. P., and Pyun, K. H., Suppression of interleukin-1 and tumor necrosis factor-α production by acanthoic acid, (−)-pimara-9(11), 15-dien-19-oic acid, and its antifibrotic effects in vivo. Cell. Immunol., 170, 212–221 (1996).
Kang, O. H., Choi, Y. A., Park, H. J., Kang, C. S., Song, B. S., Choi, S. C., Nah, Y. H., Yun, K. J., Cai, X. F., Kim, Y. H., Bae, K. H., and Lee, Y. M., Inhibition of trypsin-induced mast cell activation by acanthoic acid. J. Ethnopharmacol., 24, 105, 326–331 (2006).
Kim, J. A., Kim, D. K., Tae, J., Kang, O. H., Choi, Y. A., Choi, S. C., Kim, T. H., Nah, Y. H., Choi, S. J., Kim, Y. H., Bae, K. H., and Lee, Y. M., Acanthoic acid inhibits IL-8 production via MAPKs and NF-κB in a TNF-α-stimulated human intestinal epithelial cell line. Clin. Chim. Acta, 342, 192–202 (2004).
Kim, Y. H., Chung, B. S., and Sankawa, U., Pimaradine diterpenes from Acanthopanax koreanum. J. Nat. Prod., 51, 1080–1083 (1988).
Krawisz, J. E., Sharon, P., and Stenson, W. F., Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity. Assessment of inflammation in rat and hamster models. Gastroenterology, 87, 1344–1350 (1984).
Murthy, S. N., Cooper, H. S., Shim, H., Shah, R. S., Ibarahim, S. A., and Sedergran, D. J., Treatment of dextran sulfate sodium-induced murine colitis by intracolonic cyclosporin. Dig. Dis. Sci., 38, 1722–1734 (1993).
Okayasu, I., Hatakeyama, S., Yamada, M., Ohkusa, T., Inagaki, Y., and Nakaya, R., Novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology, 98, 694–702 (1990).
Perry, L. M. and Metzger, J., Medicinal plants of East and Southeast Asia, MIT Press, Cambridge, MA, and London, pp. 41, (1980).
Sartor, R. B., Microbial factors in the pathogenesis of Crohn’s disease, ulcerative colitis and experimental intestinal inflammation. Philadelphia: Saunders, (1999).
Shattuck-Brandt, R. L., Varilek, G. W., Radhika, A., Yang, F., Washington, M. K., and DuBois, R. N., Cyclooxygenase 2 expression is increased in the stroma of colon carcinomas from IL-10(-/-) mice. Gastroenterology, 118, 337–345 (2000).
Shiratora, Y., Aoki, S., Takada, H., Kiriyama, H., Ohto, K., Hai, K., Teraoka, H., Matano, S., Matsumoto, K., and Kamii, K., Oxygen-derived free radical generating capacity of polymorphonuclear cells in patients with ulcerative colitis. Digestion, 44, 163–171 (1989).
Simmonds, N. J. and Rampton, D. S., Inflammatory bowel disease-a radical view. Gut, 34, 865–868 (1993).
Suematsu, M., Suzuki, M., Kitahora, T., Miura, S., Suzuki, K., Hibi, T., Watanabe, M., Nagata, H., Asakura, H., and Tsuchiya, M., Increased respiratory burst of leukocytes in inflammatory bowel diseases—the analysis of free radical generation by using chemiluminescence probe. J. Clin. Lab. Immunol., 24, 125–128 (1987).
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Kang, OH., Kim, DK., Cai, XF. et al. Attenuation of experimental murine colitis by Acanthoic acid from Acanthopanax koreanum . Arch. Pharm. Res. 33, 87–93 (2010). https://doi.org/10.1007/s12272-010-2230-x
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DOI: https://doi.org/10.1007/s12272-010-2230-x