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Spirafolide from bay leaf (Laurus nobilis) prevents dopamine-induced apoptosis by decreasing reactive oxygen species production in human neuroblastoma SH-SY5Y cells

Abstract

Reactive oxygen species (ROS) are important mediators in many neurodegenerative diseases including Alzheimer’s disease and Parkinson’s disease. This study tested the neuroprotective effects of spirafolide, a compound purified from the leaves of Laurus nobilis L. (Lauraceae), against dopamine (DA)-induced apoptosis in human neuroblastoma SH-SY5Y cells. Following a 24-h exposure of cells to DA (final conc., 0.6 mM), we observed a marked increase in apoptosis, increased generation of ROS and decreased cell viability. Pretreatment of the cells for 24 h with spirafolide (0.4, 2, and 10 μM) before exposure to DA notably increased cell survival (p < 0.01) and lowered intracellular ROS levels (p < 0.01). These results indicate that spirafolide has neuroprotective effects against DA toxicity. These effects may contribute to the treatment of neurodegenerative diseases.

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Correspondence to Jongheon Shin or Woongchon Mar.

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These authors contributed equally to this work.

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Ham, A., Kim, B., Koo, U. et al. Spirafolide from bay leaf (Laurus nobilis) prevents dopamine-induced apoptosis by decreasing reactive oxygen species production in human neuroblastoma SH-SY5Y cells. Arch. Pharm. Res. 33, 1953–1958 (2010). https://doi.org/10.1007/s12272-010-1210-5

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Key words

  • Laurus nobilis
  • Spirafolide
  • Reactive oxygen species
  • Apoptosis
  • SH-SY5Y cells