Leaf and stem of Vitis amurensis and its active components protect against amyloid β protein (25–35)-induced neurotoxicity
This study investigated a methanol extract from the leaf and stem of Vitis amurensis (Vitaceae) for possible neuroprotective effects on neurotoxicity induced by amyloid β protein (Aβ) (25–35) in cultured rat cortical neurons and also for antidementia activity in mice. Exposure of cultured cortical neurons to 10 μM Aβ (25–35) for 36 h induced neuronal apoptotic death. At concentrations of 1–10 μg/mL, V. amurensis inhibited neuronal death, the elevation of intracellular calcium ([Ca2+]i) and the generation of reactive oxygen species (ROS), all of which were induced by Aβ (25–35) in primary cultures of rat cortical neurons. Memory loss induced by intracerebroventricular injection of ICR mice with 16 nmol Aβ (25–35) was inhibited by chronic treatment with V. amurensis extract (50 and 100 mg/kg, p.o. for 7 days), as measured by a passive avoidance test. Amurensin G, r-2-viniferin and trans-ɛ-viniferin isolated from V. amurensis also inhibited neuronal death, the elevation of [Ca2+]i and the generation of ROS induced by Aβ (25–35) in cultured rat cortical neurons. These results suggest that the neuroprotective effect of V. amurensis may be partially attributable to these compounds. These results suggest that the antidementia effect of V. amurensis is due to its neuroprotective effect against Aβ (25–35)-induced neurotoxicity and that the leaf and stem of V. amurensis have possible therapeutic roles for preventing the progression of Alzheimer’s disease.
Key wordsVitis amurensis Amurensin G r-2-Viniferin trans-ɛ-Viniferin Amyloid β protein Neuroprotection Memory impairment
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- Ban, J. Y., Jeon, S. Y., Bae, K., Song, K. S., and Seong, Y. H., Catechin and epicatechin from Smilacis chinae rhizome protect cultured rat cortical neurons against amyloid beta protein (25–35)-induced neurotoxicity through inhibition of cytosolic calcium elevation. Life Sci., 79, 2251–2259 (2006).CrossRefPubMedGoogle Scholar
- Cho, S. O., Ban, J. Y., Kim, J. Y., Jeong, H. Y., Lee, I. S., Song, K. S., Bae, K. H., and Seong, Y. H., Aralia cordata protects against amyloid beta protein (25–35)-induced neurotoxicity in cultured neurons and has antidementia activities. J. Pharmacol. Sci., 111, 22–32 (2009).CrossRefPubMedGoogle Scholar
- Jakab, M., Lach, S., Bacova, Z., Langeluddecke, C., Strbak, V., Schmidt, S., Lglseder, E., Paulmichl, M., Geibel, J., and Ritter, M., Resveratrol inhibits electrical activity and insulin release from insulinoma cells by block of voltagegated Ca2+ Channels and swelling-dependent Clcurrents. Cell. Physiol. Biochem., 22, 567–578 (2008).CrossRefPubMedGoogle Scholar
- Lee, E. O., Lee, H. J., Hwang, H. S., Ahn, K. S., Chae, C., Kang, K. S., Lu, J., and Kim, S. H., Potent inhibition of Lewis lung cancer growth by heyneanol A from the roots of Vitis amurensis through apoptotic and anti-angiogenic activities. Carcinogenesis, 27, 2059–2069 (2006).CrossRefPubMedGoogle Scholar
- Sano, M., Ernesto, C., Thomas, R. G., Klauber, M. R., Schafer, K., Grundman, M., Woodbur, P., Growdon, J., Cotman, C. W., Pfeiffer, E., Schneider, L. S., and Thal, L. J., A controlled trial of selegiline, alpha-tocopherol, or both as treatment for Alzheimer’s disease. The Alzheimer’s Disease Cooperative Study. N. Engl. J. Med., 336, 1216–1222 (1997).CrossRefPubMedGoogle Scholar
- Stepanichev, M. Y., Moiseeva, Y. V., Lazareva, N. A., Onufriev, M. V., and Gulyaeva, N. V., Single intracerebroventricular administration of amyloid-beta (25–35) peptide induces impairment in short-term rather than longterm memory in rats. Brain Res. Bull., 61, 197–205 (2003).CrossRefPubMedGoogle Scholar
- Wang, J., Ho, L., Zhao, W., Ono, K., Rosensweig, C., Chen, L., Humala, N., Teplow, D. B., and Pasinetti, G. M., Grapederived polyphenolics prevent A beta oligomerization and attenuate cognitive deterioration in a mouse model of Alzheimer’s disease. J. Neurosci., 28, 6388–6392 (2008).CrossRefPubMedGoogle Scholar
- Wenk, G. L., Neuropathologic changes in Alzheimer’s disease: potential targets for treatment. J. Clin. Psychiatry, 67 Suppl 3, 3–7 (2006).Google Scholar
- Yamada, K., Tanaka, T., Han, D., Senzaki, K., Kameyama, T., and Nabeshima, T., Protective effects of idebenone and alpha-tocopherol on beta-amyloid-(1–42)-induced learning and memory deficits in rats: implication of oxidative stress in beta-amyloid-induced neurotoxicity in vivo. Eur. J. Neurosci., 11, 83–90 (1999).CrossRefPubMedGoogle Scholar
- Yim, N. H., Ha, D. T., Trung, T. N., Kim, J. P., Lee, S. M., Na, M. K., Jung, H. J., Kim, H. S., Kim, Y. H., and Bae, K. H., The antimicrobial activity of compounds from the leaf and stem of Vitis amurensis against two oral pathogens. Bioorg. Med. Chem. Lett., 20, 1165–1168 (2010).CrossRefPubMedGoogle Scholar