Archives of Pharmacal Research

, Volume 33, Issue 8, pp 1261–1267 | Cite as

An experimental study of rabbit conjunctival epithelial toxicity using co-treatment with Mitomycin-C and a histone deacetylase inhibitor

  • Tae-Hyun Kim
  • Hyun Cheol Oh
  • Young Hyun Yoo
  • Jee Hyun Rho
  • Woo Chan Park
  • Hee Bae Ahn
  • Woo Jin Jeung
  • Sae Heun Rho
Research Articles Drug Actions

Abstract

This study was conducted to evaluate cytotoxicity due to co-treatment with low-dose Mitomycin-C (MMC) and the histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA) for glaucoma filtration surgery. In this study, the effect of co-treatment with MMC and SAHA to induce apoptosis in cultured conjunctival epithelial cells (CEs) in rabbit was investigated. The cytotoxic potential following co-treatment with MMC and SAHA in CEs via assay for reactive oxygen species (ROS) and lactate dehydrogenase (LDH) was also examined. Co-treatment with MMC and SAHA did not induce apoptosis in CEs. In addition, co-treatment with MMC and SAHA caused no significant alteration of ROS and LDH levels in CEs. This study therefore shows that low-dose MMC could still be used as an antimetabolite in co-treatment with SAHA to effectively inhibit fibrosis of Tenon’s capsule fibroblasts (TCFs) following glaucoma filtration surgery, while minimizing cytotoxicity in CEs.

Key words

Mitomycin-C Histone deacetylase inhibitor Suberoylanilide hydroxamic acid (SAHA) Apoptosis Glaucoma filtration surgery 

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Copyright information

© The Pharmaceutical Society of Korea and Springer Netherlands 2010

Authors and Affiliations

  • Tae-Hyun Kim
    • 1
    • 2
  • Hyun Cheol Oh
    • 3
  • Young Hyun Yoo
    • 1
  • Jee Hyun Rho
    • 3
  • Woo Chan Park
    • 3
  • Hee Bae Ahn
    • 3
  • Woo Jin Jeung
    • 3
  • Sae Heun Rho
    • 3
  1. 1.Department Anatomy and Cell BiologyDong-A University College of Medicine and Medical Science Research CenterBusanKorea
  2. 2.Institute of Tissue Regeneration Engineering (ITREN)Dankook UniversityCheonanKorea
  3. 3.Department of OphthalmologyDong-A University College of MedicineBusanKorea

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