Abstract
Human adipose-derived stem cells (hADSCs) and dental pulp-derived stem cells (hDPSCs) have been considered alternative sources of adult stem cells because of their potential to trans-differentiate into multiple cell lineages. This study investigated the possible role of gangliosides in the osteoblast differentiation of hADSCs and hDPSCs. First, we investigated characterization of hADSCs and hDPSCs using FACS analysis. Mesenchymal stem cell specific markers, CD44 and CD105, were expressed but not hematopoetic markers, CD45 and CD117 in both of hADSCs and hDPSCs. High-performance thin-layer chromatography analysis showed that increased gangliosides were associated with differentiation of hADSCs and hDPSCs into osteoblasts. RT-PCR analysis confirmed that osteoblast specific genes, ALP, BMP-2, collagen were expressed in differentiated osteoblasts, however, the another osteoblast specific gene, osteocalcin, was not expressed. When hADSCs and hDPSCs were cultured under osteoblast-differentiation conditions, alkaline phosphatase (ALP) activity was increased in comparison to hADSCs and hDPSCs. Furthermore, specifically both ALP activity and ganglioside expression increased more in hDPSCs-derived osteoblasts than hADSCs-derived osteoblasts. These results suggest that gangliosides play a more important role in regulating the osteoblast-differentiation of hDPSCs compared to hADSCs.
Similar content being viewed by others
References
Dominici, M., Le Blanc, K., Mueller, I., Slaper-Cortenbach, I., Marini, F. C., Krause, D. S., Deans, R. J., Keating, A., Prockop, D. J., and Horwitz, E. M., Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy, 8, 315–317 (2006).
El-Backly, R. M., Massoud, A. G., El-Badry, A. M., Sherif, R. A., and Maarei, M. K., Regeneration of dentine/pulp-like tissue using a dental pulp stem sell/poly(lactic-co-glycolic) acid scaffold construct in New Zealand white rabbits. Aust. Endod. J., 34, 52–67 (2008).
Ferrari, G., Cusella-De Angelis, G., Coletta, M., Paolucci, E., Stornaiuolo, A., Cossu, G., and Mavilio, F., Muscle regeneration by bone marrow-derived myogenic progenitors. Science, 279, 1528–1530 (1998).
Gronthos, S., Mankani, M., Brahim, J., Robey, P. G., Shi, S., Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc. Natl. Acad. Sci. USA, 97, 13625–13630 (2000).
Hakomori, S., Bifunctional role of glycosphingolipids, modulators for transmembrane signaling and mediators for cellular interations. J. Biol. Chem., 265, 18713–18716 (1990).
Hakomori, S., Yamamura, S., and Handa, A. K., Signal transduction through glyco(sphingo)lipid. Introduction and recent studies on glyco(sphingo)lipid-enriched microdomains. Ann. N. Y. Acad. Sci., 845, 1–10 (1998).
Hong, S. H., Gang, E. J., Jeong, J. A., Ahn, C., Hwang, S. H., Yang, I. H., Park, H. K., Han, H., and Kim, H., In vitro differentiation of human umbilical cord blood-derived mesenchymal stem cells into hepatocyte-like cells. Biochem. Biophys. Res. Commun., 330, 1153–1161 (2005).
Ikeda, E., Hirose, M., Kotobuki, N., Shimaoka, H., Tadokoro, M., Maeda, M., Hayashi, Y., Kirita, T., and Ohgushi, H., Osteogenic differentiation of human dental papilla mesenchymal stem cell. Biochem. Biophys. Res. Commun., 342, 1257–1262 (2006).
Kern, S., Eichler, H., Stoeve, J., Klüter, H., and Bieback, K., Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells, 24, 1294–1301 (2006).
Kim, S. M., Jung, J. U., Ryu, J. S., Jin, J. W., Yang, H. J., Ko, K., You, H. K., Jung, K. Y., and Choo, Y. K., Effects of gangliosides on the differentiation of human mesenchymal stem cells into osteoblasts by modulating epidermal growth factor receptors. Biochem. Biophys. Res. Commun., 371, 866–871 (2008).
Lee, D.H., Koo, D.B., Ko, K., Ko, K., Kim, S.M., Jung, J.U., Ryu, J.S., Jin, J.W., Yang, H.J., Do, S.I., Jung, K.Y., Choo, Y.K., Effects of daunorubicin on ganglioside expression and neuronal differentiation of mouse embryonic stem cells. Biochem. Biophys. Res. Commun., 362, 313–318 (2007).
Liang, L., Tao, M., Wei, C., Jinling, H., Xueli, B., Junjian, L., and Tingbo, L., Therapeutic potential and related signal pathway of adipose-derived stem cell transplantation for rat liver injury. Hepatol. Res., 39, 822–832 (2009).
Liu, Y., Li, R., and Ladisch, S., Exogenous ganglioside GD1a enhances epidermal growth factor receptor binding and dimerization. J. Biol. Chem., 279, 36481–36489 (2004).
Pittenger, M. F., Mackay, A. M., Beck, S. C., Jaiswal, R. K., Douglas, R., Mosca, J. D., Moorman, M. A., Simonetti, D. W., Craig, S., and Marshak, D. R., Multilineage potential of adult human mesenchymal stem cells. Science, 284, 143–147 (1999).
Prescott, R. S., Alsanea, R., Fayad, M. I., Johnson, B. R., Wenckus, C. S., Hao, J., John, A. S., and George, A., In vivo generation of dental pulp-like tissue by using dental pulp stem cells, a collagen scaffold, and dentin matrix protein 1 after subcutaneous transplantation in mice. J. Endod., 34, 421–426 (2008).
Pyo, H., Jeo, E., Jung, S., Lee, S. H., and Jou, I., Gangliosides activate cultured rat brain microglia. J. Biol. Chem., 274, 34584–34589 (1999).
Ryu, J. S., Ko, K., Lee, J. W., Park, S. B., Byun, S. J., Jeong, E. J., Ko, K., and Choo, Y. K., Gangliosides are involved in neural differentiation of human dental pulp-derived stem cells. Biochem. Biophys. Res. Commun., 387, 266–271 (2009).
Sanchez-Ramos, J., Song, S., Cardozo-Pelaez, F., Hazzi, C., Stedeford, T., Willing, A., Freeman, T. B., Saporta, S., Janssen, W., Patel, N., Cooper, D. R., and Sanberg, P. R., Adult bone marrow stromal cells differentiate into neural cells in vitro. Exp. Neurol., 164, 247–256 (2000).
Sato, Y., Araki, H., Kato, J., Nakamura, K., Kawano, Y., Kobune, M., Sato, T., Miyanishi, K., Takayama, T., Takahashi, M., Takimoto, R., Iyama, S., Matsunaga, T., Ohtani, S., Matsuura, A., Hamada, H., and Niitsu, Y., Human mesenchymal stem cells xenografted directly to rat liver are differentiated into human hepatocytes without fusion. Blood, 106, 756–763 (2005).
Stevens, A., Zuliani, T., Olejnik, C., LeRoy, H., Obriot, H., Kerr-Conte, J., Formstecher, P., Bailliez, Y., and Polakowska, R. R., Human dental pulpstem cells differentiate into neural crest-derived melanocvtes and have label-retaining and sphere-forming abilities. Stem Cells Dev., 17, 1175–1184 (2008).
Yamamoto, A., Haraguchi, M., Yamashiro, S., Fukumoto, S., Furukawa, K., Takamiya, K., Atsuta, M., and Shiku, H., Heterogeneity in the expression pattern of two ganglioside synthase genes during mouse brain development. J. Neurochem., 66, 26–34 (1996).
Yu, R. K., Macala, L. J., Taki, T., Weinfield, H. M., Yu, F. S., Developmental changes in ganglioside composition and synthesis in embryonic rat brain. J. Neurochem., 50, 1825–1829 (1988).
Yu, R. K., Development regulation of ganglioside metabolism. Prog. Brain Res., 101, 31–44 (1994).
Zhu, Y., Liu, T., Song, K., Fan, X., Ma, X., and Cui, Z., Adipose-derived stem cell: a better stem cell than BMSC. Cell Biochem. Funct., 26, 664–675 (2008).
Author information
Authors and Affiliations
Corresponding author
Additional information
These authors contributed equally to this work.
Rights and permissions
About this article
Cite this article
Lee, S.H., Ryu, JS., Lee, JW. et al. Comparison of ganglioside expression between human adipose- and dental pulp-derived stem cell differentiation into osteoblasts. Arch. Pharm. Res. 33, 585–591 (2010). https://doi.org/10.1007/s12272-010-0413-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12272-010-0413-0