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Galactosylated chitosan-g-PEI/DNA complexes-loaded poly(organophosphazene) hydrogel as a hepatocyte targeting gene delivery system

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Abstract

Hydrogels are widely used in drug delivery systems because they can control the release and thereby enhance the efficiency of locally delivered bioactive molecules such as therapeutic drugs, proteins, or genes. For gene delivery, localized release of plasmid DNA or polymer/DNA complexes can transfect cells and produce sustained protein production. We tested the galactosylated chitosan-graft-polyethylenimine (GC-g-PEI)/DNA complexes-loaded poly(organophosphazene) thermosensitive biodegradable hydrogel as a hepatocyte targeting gene delivery system. The poly(organophosphazene) hydrogel loaded with GC-g-PEI/DNA complexes showed low cytotoxicity and higher transfection efficiency than PEI/DNA complexes, as well as good hepatocyte specificity in vitro and in vivo. Our results indicate that poly(organophosphazene) hydrogels loaded with GC-g-PEI/DNA complexes may be a safe and efficient hepatocyte targeting gene delivery system.

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Authors and Affiliations

  1. College of Veterinary Medicine, Seoul National University, Seoul, 151-742, Korea

    Hu-Lin Jiang & Myung-Haing Cho

  2. Department of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea

    You-Kyoung Kim, Yong-Mei Jin, Rohidas Arote & Chong-Su Cho

  3. Division of Life Science, Korea Institute of Science and Technology, Seoul, 130-650, Korea

    Sun-Mi Lee, Mi-Ran Park & Soo-Chang Song

  4. Department of Nuclear Medicine, Research Institute of Clinical Medicine, Chonbuk National University School of Medicine, Jeonju, 561-712, Korea

    Eun-Mi Kim & Hwan-Jeong Jeong

Authors
  1. Hu-Lin Jiang
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  2. You-Kyoung Kim
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  3. Sun-Mi Lee
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  4. Mi-Ran Park
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  5. Eun-Mi Kim
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  6. Yong-Mei Jin
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  7. Rohidas Arote
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  8. Hwan-Jeong Jeong
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  9. Soo-Chang Song
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  10. Myung-Haing Cho
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  11. Chong-Su Cho
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Correspondence to Chong-Su Cho.

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Jiang, HL., Kim, YK., Lee, SM. et al. Galactosylated chitosan-g-PEI/DNA complexes-loaded poly(organophosphazene) hydrogel as a hepatocyte targeting gene delivery system. Arch. Pharm. Res. 33, 551–556 (2010). https://doi.org/10.1007/s12272-010-0409-9

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  • DOI: https://doi.org/10.1007/s12272-010-0409-9

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