Archives of Pharmacal Research

, Volume 33, Issue 2, pp 309–316 | Cite as

Assessment of the anti-angiogenic, anti-inflammatory and antinociceptive properties of ethyl vanillin

  • Hyun-Joo Jung
  • Yun Seon Song
  • Kyunghoon Kim
  • Chang-Jin LimEmail author
  • Eun-Hee ParkEmail author
Research Articles Drug Actions


The present work aimed to assess novel pharmacological properties of ethyl vanillin (EVA) which is used as a flavoring agent for cakes, dessert, confectionary, etc. EVA exhibited an inhibitory activity in the chorioallantoic membrane angiogenesis. Anti-inflammatory activity of EVA was convinced using the two in vivo models, such as vascular permeability and air pouch models in mice. Antinociceptive activity of EVA was assessed using acetic acid-induced writhing model in mice. EVA suppressed production of nitric oxide and induction of inducible nitric oxide synthase in the lipopolysaccharide (LPS)-activated RAW264.7 macrophage cells. However, EVA could not suppress induction of cyclooxygenase-2 in the LPS-activated macrophages. EVA diminished reactive oxygen species level in the LPS-activated macrophages. EVA also suppressed enhanced matrix metalloproteinase-9 gelatinolytic activity in the LPSactivated RAW264.7 macrophage cells. EVA at the used concentrations couldn’t diminish viability of the macrophage cells. Taken together, the anti-angiogenic, anti-inflammatory and anti-nociceptive properties of EVA are based on its suppressive effect on the production of nitric oxide possibly via decreasing the reactive oxygen species level.

Key words

Anti-angiogenic Anti-inflammatory Antinociceptive Ethyl vanillin Nitric oxide Reactive oxygen species 


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Copyright information

© The Pharmaceutical Society of Korea and Springer Netherlands 2010

Authors and Affiliations

  1. 1.Brain Korea 21 Project for Medical Science, Department of AnatomyYonsei University College of MedicineSeoulKorea
  2. 2.College of PharmacySookmyung Women’s UniversitySeoulKorea
  3. 3.Division of Life Sciences and Research Institute of Life SciencesKangwon National UniversityChuncheonKorea
  4. 4.Division of Life Sciences, College of Natural SciencesKangwon National UniversityChuncheonKorea

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