Abstract
The aim is to study the effect of dimethylsulfoxide-soluble smoking particles (DSP) and nicotine on the contractility of rat mesenteric artery. The superior mesenteric artery segments were cultured with DSP or nicotine for 24 h. The vascular contractibility was recorded with myograph system. DSP 0.4 mL/L and nicotine 0.48 and 0.96 mg/L shifted the concentration-contractile curves induced by sarafotoxin 6c, a selective agonist for ETB receptor toward the left with increased Emax. DSP 0.4 mL/L and nicotine 0.96 mg/L shifted ETA receptor-mediated the concentration-contractile curves toward the left with increased Emax. However, nicotine 0.06 mg/L which is the equivalent concentration of nicotine in DSP 0.4 mL/L did not affect the curves and the Emax mediated with ETA receptor and ETB receptor. DSP 0.2 and 0.4 mL/L shifted the concentration-contractile curves induced by noradrenaline toward the right with decreased Emax. Neither did nicotine 0.06 and 0.96 mg/L. Both DSP and nicotine shifted the concentration-contractile curves induced by 5-hydroxytryptamine (5-HT) toward the right parallely. DSP changed the phenotypes towards an increased efficacy of ETA receptor and ETB receptor, and a reduced efficacy of 5-HT receptor and α-adrenocceptor. The effects of DSP on ETB receptor, ETA receptor and α-adrenocceptor were independent of nicotine. The effect on 5-HT receptor was responsible to nicotine.
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References
Alm, R., Edvinsson, L., and Malmsjö, M., Organ culture: a new model for vascular endothelium dysfunction. BMC Cardiovasc. Disord., 2, 8–12 (2002).
Bergdahl, A., Valdemarsson, S., Adner, M., Sun, X. Y., Hedner, T., and Edvinsson, L., Enhanced endothelin-1-induced contractions in mesenteric arteries from rats with congestive heart failure: role of ET (B) receptors. Eur J Heart Fail, 3, 293–299 (2001).
Eguchi, S., Miyashita, S., Kitamura, Y., and Kawasaki, H., Alpha3beta4-nicotinic receptors mediate adrenergic nerveand peptidergic (CGRP) nerve-dependent vasodilation induced by nicotine in rat mesenteric arteries. Br. J. Pharmacol., 151, 1216–1223 (2007).
Gourlay, S. G. and Benowitz, N. L., Arteriovenous differences in plasma concentration of nicotine and catecholamines and related cardiovascular effects after smoking, nicotine nasal spray, and intravenous nicotine. Clin. Pharmacol. Ther[J]., 62, 453–463 (1997).
Hansen-Schwartz, J., Hoel, N. L., Xu, C. B., Svendgaard, N. A., and Edvinsson, L., Subarachnoid hemorrhage-induced upregulation of the 5-HT1B receptor in cerebral arteries in rats. J. Neurosurg., 99, 115–120 (2003a).
Hansen-Schwartz, J., Hoel, N. L., Zhou, M., Xu, C. B., Svendgaard, N. A., and Edvinsson, L., Subarachnoid hemorrhage enhances endothelin receptor expression and function in rat cerebral arteries. Neurosurgery, 52, 1188–1195 (2003b).
Hawkins, B. T., Brown, R. C., and Davis, T. P., Smoking and ischemic stroke: a role for nicotine? Trends Pharmacol. Sci., 23, 78–82 (2002).
Henningfield, J., Stapleton, J. M., Benowitz, N. L., Grayson, R. F., and London, E. D., Higher levels of nicotine in arterial than in venous blood after cigarette smoking. Drug Alcohol Depend[J]., 33, 23–29 (1993).
Kiyohara, Y., Ueda, K., and Fujishima, M., Smoking and cardiovascular disease in the general population in Japan. J. Hypertens. Suppl., 8, 9–15 (1990).
Rose, J. E., Behm, F. M., Westman, E. C., and Coleman, R. E., Arterial nicotine kinetics during cigarette smoking and intravenous nicotine administration: implications for addiction. Drug Alcohol Depend[J]., 56, 99–107 (1999).
Shiraki, H., Kawasaki, H., Tezuka, S., Nakatsuma, A., and Kurosaki, Y., Endogenous calcitonin gene-related peptide (CGRP) mediates adrenergic-dependent vasodilation induced by nicotine in mesenteric resistance arteries of the rat. Br. J. Pharmacol., 130, 1083–1091 (2000).
Stenman, E., Malmsjo, M., Uddman, E., Gido, G., Wieloch, T., and Edvinsson, L., Cerebral ischemia upregulates vascular endothelin ET (B) receptors in rat. Stroke, 33, 2311–2316 (2002).
Xu, C. B., Pessah-Rasmussen, H., and Stavenow, L., Interactions between cultured bovine arterial endothelial and smooth muscle cells: effects of injury on the release of growth stimulating and growth inhibiting substances. Pharmacol. Toxicol., 69, 195–200 (1991).
Xu, C. B., Stavenow, L., and Pessah-Rasmussen, H., Interactions between cultured bovine arterial endothelial and smooth muscle cells; further studies on the effects of injury and modification of the consequences of injury. Artery, 20, 163–179 (1993).
Zhang, J. Y., Cao, Y. X., Xu, C. B., and Lars, E., Lipidsoluble smoke particles damage endothelial cells and reduce endothelium-dependent dilatation in rat and man. BMC Cardiovasc. Disord[J]., 6, 3 (2006).
Zhang, W., Cao, Y. X., He, J. Y., and Xu, C. B., Down-regulation of alpha-adrenoceptor expression by lipid-soluble smoke particles through transcriptional factor nuclear factor-kappaB pathway. Basic Clin. Pharmacol. Toxicol., 101, 401–406 (2007).
Zhu, Z. H., Liu, J. L., Zhang, J. X., and He, Y. Q., Determination of total nicotine and nicotine by Spectrophotometry. Journal of Environment and Health, 14, 230–232 (1997).
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Zhang, JY., Cao, L., Zheng, XH. et al. Dimethylsulfoxide-soluble smoking particles and nicotine affect vascular contractibility. Arch. Pharm. Res. 32, 1475–1481 (2009). https://doi.org/10.1007/s12272-009-2019-y
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DOI: https://doi.org/10.1007/s12272-009-2019-y