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Effects of dimyristoylphosphatidylethanol and ethanol on thickness of neuronal membrane lipid bilayers

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Abstract

The aim of this study was to provide a basis for examining the molecular mechanism for the pharmacological action of ethanol. Energy transfer between the surface fluorescent probe 1-anilinonaphthalene-8-sulfonic acid and hydrophobic fluorescent probe 1,3-di(1-pyrenyl)propane was used to examine the effect of both dimyristoylphosphatidylethanol (DMPEt) and ethanol on the thickness (D) of the synaptosomal plasma membrane vesicles (SPMV) isolated from the bovine cerebral cortex. The thickness (D) of the intact SPMV was 1.044 ± 0.008 (arbitrary units, n=5) at 37°C (pH 7.4). Both DMPEt and ethanol decreased the thickness of the SPMV lipid bilayer in a dose-dependent manner with a significant decrease in thickness observed at 5 µM and 25 mM, respectively. It was assumed that both ethanol and DMPEt cause interdigitation in the SPMV lipid bilayers. The effects of ethanol on the neuronal membranes were attributed to its direct and indirect actions. The indirect action of ethanol refers to the action of phosphatidylethanol, which is an ethanol abnormal metabolite, on the neuronal membranes. The decrease in membrane thickness by both DMPEt and ethanol might be responsible for some, but not all of its anesthetic actions.

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References

  • Alling, C., Gustavsson, L., Månsson, J-. E., Benthin, G., and Anggård, E., Phosphatidylethanol formation in rat organs after ethanol treatment. Biochim. Biophys. Acta., 793, 119–122 (1984).

    CAS  PubMed  Google Scholar 

  • Armbrecht, H. J., Wood, W. G., Wise, R. W., Walsh, J. B., Thomas, B. N., and Strong, R., Ethanol-induced disordering of membranes from different age groups of C57BL/6NNIA mice. J. Pharmacol. Exp. Ther., 226, 387–391 (1983).

    CAS  PubMed  Google Scholar 

  • Bae, M. K., Jeong, D. K., Park, N. S., Lee, C. H., Cho, B. H., Jang, H. O., and Yun, I., The effect of ethanol on the physical properties of neuronal membranes. Mol. Cells, 19, 356–364 (2005).

    CAS  PubMed  Google Scholar 

  • Bangham, A. D. and Mason, W., The effect of some general anesthetics on the surface potential of lipid monolayers. Br. J. Pharmacol., 66, 259–265 (1979).

    CAS  PubMed  Google Scholar 

  • Chin, J. H. and Goldstein, D. B., Effects of low concentrations of ethanol on the fluidity of spin-labeled erythrocyte and brain membranes. Mol. Pharmacol., 13, 435–441 (1977a).

    CAS  PubMed  Google Scholar 

  • Chin, J. H. and Goldstein, D. B., Drug tolerance in biomembranes: a spin label study of the effects of ethanol. Science, 196, 684–685 (1977b).

    Article  CAS  PubMed  Google Scholar 

  • Chin, J. H. and Goldstein, D. B., Membrane-disordering action of ethanol: variation with membrane cholesterol content and depth of the spin label probe. Mol. Pharmacol., 19, 425–431 (1981).

    CAS  PubMed  Google Scholar 

  • Chin, J. H. and Goldstein, D. B., Cholesterol blocks the disordering effects of ethanol in biomembranes. Lipids, 19, 929–935 (1984).

    Article  CAS  PubMed  Google Scholar 

  • Chung, I. K., Kang, J. S., and Yun, I., The effect of n-alkanols on the lateral diffusion of synaptosomal plasma membrane vesicles isolated from bovine cerebral cortex. Korean J. Pharmacol., 29, 157–163 (1993).

    CAS  Google Scholar 

  • Davidson, F. M. and Long, C., The structure of the naturally occurring phosphoglycerides. 4. Action of cabbage-leaf phospholipase D on ovolecithin and related substances. Biochem. J., 69, 458–466 (1958).

    CAS  PubMed  Google Scholar 

  • Dobretsov, G. E., Spirin, M. M., Chekrygin, O. V., Karamansky, I. M., Dmitriev, V. M., Vladimirov, Y. A., A fluorescence study of apolipoprotein localization in relation to lipids in serum low density lipoproteins. Biochim. Biophys. Acta., 710, 172–180 (1982).

    CAS  PubMed  Google Scholar 

  • Eisinger, J. and Flores, J., The relative locations of intramembrane fluorescent probes and of the cytosol hemoglobin in erythrocytes, studied by transverse resonance energy transfer. Biophys. J., 37, 6–7 (1982).

    Article  CAS  PubMed  Google Scholar 

  • Goldstein, D. B. and Chin, J. H., Interaction of ethanol with biological membranes. Fed. Proc., 40, 2073–2076 (1981).

    CAS  PubMed  Google Scholar 

  • Harris, R. A. and Schroeder, F., Ethanol and the physical properties of brain membranes. Fluorescence studies. Mol. Pharmacol., 20, 128–137 (1981).

    CAS  PubMed  Google Scholar 

  • Jang, H. O., Jeong, D. K., Ahn, S. H., Yoon, C. D., Jeong, S. C., Jin, S. D., and Yun, I., Effects of chlorpromazine·HCl on the structural parameters of bovine brain membranes. J. Biochem. Mol. Biol., 37, 603–611 (2004a).

    CAS  PubMed  Google Scholar 

  • Jang, H. O., Shin, H. G., and Yun, I., Effects of dimyristoylphosphatidylethanol on the structural parameters of neuronal membranes. Mol. Cells, 17, 485–491 (2004b).

    CAS  PubMed  Google Scholar 

  • Kang, J. -S., Choi, Ch. -M., and Yun, I., Effects of ethanol on lateral and rotational mobility of plasma membrane vesicles isolated from cultured mouse myeloma cell line Sp2/0-Ag14. Biochim. Biophys. Acta., 1281, 157–163 (1996).

    Article  PubMed  Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193, 265–275 (1951).

    CAS  PubMed  Google Scholar 

  • Majchrowicz, E. and Mendelson, J. H., Blood methanol concentrations during experimentally induced ethanol intoxication in alcoholics. J. Pharmacol. Exp. Ther., 179, 293–300 (1971).

    CAS  PubMed  Google Scholar 

  • Omodeo-Salê, M. F., Cestaro, B., Mascherpa, A., Monti, D., and Masserini, M., Enzymatic synthesis and thermotropic behavior of phosphatidylethanol. Chem. Phys. Lipids, 50, 135–142 (1989).

    Article  Google Scholar 

  • Omodeo-Salê, M. F., Lindi, C., Palestini, P., and Masserini, M., Role of phosphatidylethanol in membranes. Effects on membrane fluidity, tolerance to ethanol, and activity of membrane-bound enzymes. Biochemistry, 30, 2477–2482(1991).

    Article  PubMed  Google Scholar 

  • Yun, I. and Kang, J. -S., The general lipid composition and aminophospholipid asymmetry of synaptosomal plasma membrane vesicles isolated from bovine cerebral cortex. Mol. Cells, 1, 15–20 (1990).

    CAS  Google Scholar 

  • Yun, I., Kim, Y. -S., Yu, S. -H., Chung, I. -K., Kim, I. -S., Baik, S. -W., Cho, G. -J., Chung, Y. -Z., Kim, S. -H., and Kang, J. -S., Comparison of several procedures for the preparation of synaptosomal plasma membrane vesicles. Arch. Pharm. Res., 13, 325–329 (1990).

    Article  Google Scholar 

  • Yun, I., Lee, S. -H., and Kang, J. -S., Effects of ethanol on lateral and rotational mobility of plasma membrane vesicles isolated from cultured Mar 18.5 hybridoma cells. J. Membr. Biol., 138, 221–227 (1994).

    CAS  PubMed  Google Scholar 

  • Yun, I., Yang, M. -S., Kim, I. -S., and Kang, J. -S., Bulk vs. transbilayer effects of ethanol on the fluidity of the plasma membrane vesicles of cultured Chinese hamster ovary cells. Asia. Pacific. J. Pharmacol., 8, 9–16 (1993).

    CAS  Google Scholar 

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Authors and Affiliations

  1. Departments of Dental Pharmacology and Biophysics, School of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Yangsan, 626-870, Korea

    Jun-Seop Park, Eun-Jong Choi, Dong-Soo Jeong, Hak-Jin Yang, Kyoung-Phil Kim, Kuk-Kyung Son, Hyun-Wook Lee, Min-Jung Kang, Sang-Min Kim, Hye-Ock Jang & Il Yun

  2. Oral and Maxillofacial Surgery and Clinical Pharmacology, School of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Yangsan, 626-870, Korea

    In-Kyo Chung

  3. Oral Physiology and Molecular Biology, School of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Yangsan, 626-870, Korea

    Moon-Kyung Bae

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  1. Jun-Seop Park
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  2. Eun-Jong Choi
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  3. Dong-Soo Jeong
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  8. Min-Jung Kang
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  9. Sang-Min Kim
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  10. In-Kyo Chung
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  11. Moon-Kyung Bae
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  12. Hye-Ock Jang
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  13. Il Yun
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Corresponding authors

Correspondence to Hye-Ock Jang or Il Yun.

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Park, JS., Choi, EJ., Jeong, DS. et al. Effects of dimyristoylphosphatidylethanol and ethanol on thickness of neuronal membrane lipid bilayers. Arch. Pharm. Res. 32, 1469–1473 (2009). https://doi.org/10.1007/s12272-009-2018-z

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  • DOI: https://doi.org/10.1007/s12272-009-2018-z

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