Abstract
The present study investigated antidepressant-like interaction of five herb polysaccharide fractions from Banxia-Houpu decoction, composed of rhizome pinelliae (PRP), magnolia bark (PMB), poria (PPO), ginger rhizome (PGR) and folium perillae (PFP) by a L16(25) orthogonal array design with a two-factor interaction in the tail suspension and forced swimming and tests (TST, FST) in mice. Serotonin (5-HT) and dopamine (DA) levels in whole mouse brain were simultaneously examined after the FST exposure. Polysaccharides PMB, PPO, PFP, and interactions of PRP × PGR, PMB × PFP, PPO × PFP reduced immobility in the mouse FST. Furthermore, PMB and PPO were two principal components of polysaccharides elevating brain 5-HT levels. PFP was an adjuvant component elevating brain DA levels. These results demonstrated that ineffective PRP exhibited antidepressant-like effects when combined with another ineffective PGR, and PFP played a role in assisting effects of PMB and PPO. Therefore, PMB × PFP and PPO × PFP interactions on antidepressant-like effects were the cores in compatibility of polysaccharides from Banxia-Houpu decoction by attenuating abnormalities in the serotonergic and dopaminergic system functions in animal models of depression.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bai, F., Li, X., Clay, M., Lindstrom, T., and Skolnick, P., Intra and interstrain differences in models of “behavioral despair”. Pharmacol. Biochem. Behav., 70, 187–192 (2001).
Delgado, P. and Moreno, F., Antidepressants and the brain. Int. Clin. Psychopharmacol., 14, S9–16 (1999)
Guo, Y. S., Kong, L. D., Wang, Y. M., and Huang, Z. Q., Antidepressant evaluation of polysaccharides from a Chinese herbal medicine Banxia-houpu decoction. Phytother. Res., 18, 204–207 (2004).
Lan, W. G., Wong, M. K., Chen, N., and Sin, Y. M., Orthogonal array design as a chemometric method for the optimization of analytical procedures. Part 1. Two-level design and its application in microwave dissolution of biological samples. Analyst, 119, 1659–1667 (1994).
Nutt, D. J., Relationship of neurotransmitters to the symptoms of major depressive disorder. J. Clin. Psychiatry, 69, 4–7 (2008).
Porsolt, R. D., Bertin, A., and Jalfre, M., Behavioral despair in mice: a primary screening test for antidepressants. Arch. Int. Pharmacodyn. Ther., 229, 327–336 (1977).
Renard, C. E., Dailly, E., David, D. J., Hascoet, M., and Bourin, M., Monoamine metabolism changes following the mouse forced swimming test but not the tail suspension test. Fundam. Clin. Pharmacol., 17, 449–455 (2003).
Rénéric, J. P. and Lucki, I., Antidepressant behavioral effects by dual inhibition of monoamine reuptake in the rat forced swimming test. Psychopharmacology (Berl.), 136, 190–197 (1998).
Stahl, S. M., Essential Psychopharmacology: Neuroscientific Basis and Practical Applications. Cambridge University Press, Cambridge, pp. 283–293 (2000).
Staub, A. M., Removal of proteins. Sevag method. Methods in Carbohyd. Chem., 5, 5–6 (1965).
Steru, L., Chermat, R., Thierry, B., and Simon, P., The tail suspension test: a newmethod for screening antidepressants in mice. Psychopharmacology (Berl.), 85, 367–370 (1985).
Wang, Y. M., Kong, L. D., and Chen, Y., Behavioural and biochemical effects of fractions prepared from Banxia Houpu decoction in depression models in mice. Phytother. Res., 19, 526–529 (2005).
Wang, L., Zhou, G. B., Liu, P., Song, J. H., Liang, Y., Yan, X. J., Xu, F., Wang, B. S., Mao, J. H., Shen, Z. X., Chen, S. J., and Chen, Z., Dissection of mechanisms of Chinese medicinal formula Realgar-Indigo naturalis as an effective treatment for promyelocytic leukemia. Proc. Natl. Acad. Sci. USA, 105, 4826–4831 (2008).
Wei, F. H., Luo, J. B., Yu, L. Z., and Mo, Z. X., The effects of herba ephedrae decoction and other compatibilities on independent locomotion of the mice. Lishizhen Medicine and Materia Medica Res., 17, 988–989 (2006).
Welch, A. S. and Welch, B. L., Solvent extraction method for simultaneous determination of norepinephrine, dopamine, serotonin, and 5-hydroxyindoleacetic acid in a single mouse brain. Anal. Biochem., 30, 161–179 (1969).
Yi, L. T., Xu, Q., Li, Y. C., Yang, L., and Kong, L. D., Antidepressant-like synergism of extracts from magnolia bark and ginger rhizome alone and in combination in mice. Prog. Neuro-Psychopharmacol. Biol. Psychiatry, 33, 616–624 (2009).
Zhang, L., Hu, H. B., Huang, C. Y., Han, Y., and Xia, C., Studies on the composition of polysaccharide from bupleurum scorzonerifolium. Chinese Traditional Herbal Drug, 31, 647–648 (2000).
Zhang, L. C., Clinical Examples of “Banxia Houpo Decoction”. Shanghai J. TCM., 37, 19–20 (2003).
Zhang, W. Y., Li, J. M., Zhu, J. X., Shi, Z. Q., Wang, Y., and Kong, L. D., Chinese medicine Banxia-houpu decoction regulates c-fos expression in the brain regions in chronic mild stress model in rats. Phytother. Res., 18, 200–203 (2004).
Zhu, Q. S., Zhao, Y. Z., Zhang, M. Y., Wang, L. M., Bai, X., and Yang, Yu., Anti-diarrhea effects of Baishushaoyao powder and its compatibility: an experimental study. Heilongjiang Med. Pharmacy, 30, 14–15 (2007).
Author information
Authors and Affiliations
Corresponding author
Additional information
These authors contributed equally to the work.
Rights and permissions
About this article
Cite this article
Yi, LT., Zhang, L., Ding, AW. et al. Orthogonal array design for antidepressant compatibility of polysaccharides from Banxia-Houpu decoction, a traditional Chinese herb prescription in the mouse models of depression. Arch. Pharm. Res. 32, 1417–1423 (2009). https://doi.org/10.1007/s12272-009-2011-6
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12272-009-2011-6