Abstract
The antimicrobial effect of solvent extracts from the rhizome of a thick-stemmed wood fern (Dryopteris crassirhizoma) was evaluated and its phloroglucinol components, flavaspidic acids PB and AB. Flavaspidic acids PB and AB were isolated from the D. crassirhizoma rhizomes by methanol extraction, followed by silica gel and Sephadex LH-20 column chromatography. The chemical structures were characterized by spectral techniques, including ESI-MS, UV, 1H- and 13C-NMR spectrum analysis. When the antimicrobial activity of the extracts and compounds was tested by the paper disc method, the extracts and compounds were highly active against Gram-positive bacteria, such as methicillin-resistant Staphylococcus aureus KCTC 1928 (a MRSA bacterium), Streptococcus mutans and Bacillus subtilis. The extracts and compounds were not active against fungi and chlorella. Our study revealed that the antibacterial activity of samples from D. crassirhizoma was mainly related to the flavaspidic acids.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Dharmananda, S., SARS AND CHINESE MEDICINE. How the Chinese People and Institutions Responded with Herbs: http://www.itmonline.org/arts/sars.htm (2003).
Do, D. S., Antibacterial components of Dryopteris crassirhizoma against a Streptococcus mutans OMZ 176. MS thesis, Chungnam National University. Daejeon, Korea (1993).
Govind, J. K., Harukuni, T., Takao, K., Midori, T., Junko, T., and Hoyoku, N., Anti-tumor promoting activity of Dryopteris phlorophenone derivatives. Cancer Letters, 105, 161–165 (1996).
Hideo, N., Munehisa, A., Akio, F., Saburo, K., and Katsuhiko. O., Inhibition of HIV-reverse transcriptase activity by some phloroglucinol derivatives. FEBS Letters, 286, 83–85 (1991).
Kang, K. A., Lee, K. H., Chae, S., Zhang, R., Jung, M. S., Ham, Y. M., Baik, J. S., Lee, N. H., and Hyun, J. W., Cytoprotective effect of phloroglucinol on oxidative stress induced cell damage via catalase activation. J. Cell Biochem., 97, 609–620 (2006).
Lee, S. M., Na, M. K. An, R. B., Min, B. S., and Lee, H. K., Antioxidant activity of two phloroglucinol derivatives from Dryopteris crassirhizoma. Biol. Pharm. Bull., 26, 1354–1356 (2003).
Mathekga, A. D. M., Meyer, J. J. M., Horn, M. M., and Drewes, S. E., An acylated phloroglucinol with antimicrobial properties from Helichrysum caespititium. Phytochemistry, 53, 93–96 (2000).
Min, B. S., Tomiyama, M., Ma, C. M., Nakamura, N., and Hattori, M., Kaempferol Acetylrhamnosides from the rhizome of Dryopteris crassirhizoma and their inhibitory effects on three different activities of human immunodeficiency Virus-I reverse transcriptase. Chem. Pharm. Bull., 49, 546–550 (2001).
Na, M., Jang, J., Min, B. S., Lee, S. J., Lee, M. S., Kim, B. Y. Oh, W. K., and Ahn, J. S., Fatty acid synthase inhibitory activity of acylphloroglucinols isolated from Dryopteris crassirhizoma. Bioorg. Med. Chem. Lett., 16, 4738–4742 (2006).
Namba, T., Tsunezuka, M., and Hattori, M., Dental caries prevention by traditional Chinese medicines. Planta Medica, 44, 100–106 (1982).
Namba, T., The encyclopedia of Wakan-Yaku with Color Picture Vol. I. Osaka, Japan: Hoikusha Co., Ltd., pp. 142–144 (1993).
Noro, Y., Okuda, K., Hisada, S., Inagaki, I., Tanaka, T., and Yokohashi, H., Dryocrassin: A new acylphloroglucinol from Dryopteris crassirhizoma. Phytochemistry, 12, 1491–1493 (1973).
Rie, I., Masakazu, H., Koichi, S., Munehisa, A., and Susumu, K., Inhibitory effects of phloroglucinol derivatives from Mallotus japonicus on nitric oxide production by a murine macrophage-like cell line, RAW 264.7, activated by lipopolysaccharide and interferon-γ. Biochimica et Biophysica Acta — General Subjects, 1568, 74–82 (2001).
Widen, C. -J., Fraser-Jenkins, C. R., Reichstein, T., Gibby, M., and Sarvela, J., Phloroglucinol derivatives in Dryopteris sect. Fibrillosae and related taxa (Pteridophyta, Dryopteridaceae). Ann. Bot. Fennici, 33, 69–100 (1996).
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Lee, H.B., Kim, J.C. & Lee, S.M. Antibacterial activity of two phloroglucinols, flavaspidic acids AB and PB, from Dryopteris crassirhizoma . Arch. Pharm. Res. 32, 655–659 (2009). https://doi.org/10.1007/s12272-009-1502-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12272-009-1502-9