Abstract
The hexane, chloroform, ethyl acetate and aqueous-soluble fractions from leaves of Maytenus robusta (Celastraceae) were evaluated for their protective actions against ethanol-induced gastric lesions in rats. The treatment with all fractions (150 mg/kg) and omeprazol (30 mg/kg) significantly reduced the lesion index, the total lesion area, and the percentage of lesion, in comparison with the control group (p<0.05). Since the ethyl acetate-soluble fraction was found to be most active in the pylorus ligated model, this fraction was further investigated and resulted in the isolation of triterpene 3,15-dioxo-21α-hydroxy friedelane. The triterpene was evaluated in the HCl/ethanol-induced ulcer model in mice. In this assay, both the groups treated with 3,15-dioxo-21α-hydroxy friedelane and omeprazol, at a dose of 30 mg/kg, presented a significant reduction in lesion index, total lesion area, and in the percentage of the lesion, when compared with the control group (p<0.05). The result suggests that the antiulcer effect observed in the extract and fractions may be attributed, at least in part, to this compound. Further experiments are underway to determine which antiulcer mechanisms involved in gastroprotection.
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Andrade, S. F., Antoniolli, D., Comunello, E., Cardoso, L. G. V., Carvalho, J. C. T., and Bastos, J. K., Antiulcerogenic activity of crude extract, fractions and populnoic acid isolated from Austroplenckia populnea (Celastraceae). Z. Naturforsch., 61c, 329–333 (2006).
Andrade, S. F., Lemos, M., Comunello, E., Noldin, V. F., Cechinel Filho, V., and Niero, R., Evaluation of the antiulcerogenic activity of Maytenus robusta (Celastraceae) in different experimental ulcer models. J. Ethnopharmacol., 113, 252–257 (2007).
Balbach, A., A flora nacional na medicina doméstica. 2nd Ed. A edificação do Lar (Ed.) São Paulo, pp.34, (1980).
Bruning, R. and Wagner, H., Übersicht über die celastraceeninhaltsstoffe: Chemie, chemotaxonomie, biosynthese, pharmakologie. Phytochemistry, 17, 1821–1858 (1978).
Chan, F. K. and Leung, W. K., Peptic ulcer disease. Lancet, 360, 933–941 (2002).
Cipriani, T. R., Mellinger, C. G., De Souza, L. M., Baggio, C. H., Freitas, C. S., Marques, M. C. A., Gorin, P. A. J., Sassaki, G. L., and Iacomini, M., A Polysaccharide from a Tea (Infusion) of Maytenus ilicifolia Leaves with Anti-ulcer Protective Effects. J. Nat. Prod., 69, 1018–1021 (2006).
Corrêa, M. P., Dicionário das plantas úteis do Brasil e das exóticas cultivadas. Ministério da Agricultura, Ed. Instituto Brasileiro de Desenvolvimento Florestal, Rio de Janeiro, pp. 134–135 (1984).
Gonzalez, J. G., Monache, G. D., Monache, F. D., and Marini-Bettolò, G. B., Chuchuasca — a drug used in folk medicine in the Amazonian and Andean areas. A chemical study of Maytenus laevis. J. Ethnopharmacol., 5, 73–77 (1982).
Gonzalez, F. G., Portela, T. Y., Stipp, E. J., and Di Stasi, L. C., Antiulcerogenic and analgesic effects of Maytenus aquifolium, Sorocea bomplandii and Zolernia ilicifolia. J. Ethnopharmacol., 77, 41–47 (2001).
Hoogerwerf, W. A. and Pasricha, P. J., Pharmacotherapy of gastric acidity, peptic ulcers, and gastroesophageal reflux disease. In: Brunton, L. L., Lazo, J. S., Parker, K. L., (Eds.). Goodman & Gilman’s The Pharmacological basis of therapeutics, 11th ed. McGraw-Hill Medical publishing division, NewYork, pp.967–981 (2006).
Itokawa, H., Shirota, O., Ikuta, H., Morita, H., Takeya, K., and Itaka, Y., Triterpenes from Maytenus ilicifolia. Phytochemistry, 30, 3713–3716 (1991).
Jorge, R. M., Leite, J. P. V., Oliveira, A. B., and Tagliati, C. A., Evaluation of antinociceptive, anti-inflammatory and antiulcerogenic activities of Maytenus ilicifolia. J. Ethnopharmacol., 94, 93–100 (2004).
Kinoshita, M., Tsunehisa, N., and Tamaki, H., Effect of a combination of ecabet sodium and cimetidine on experimentally induced gastric-lesions and gastric-mucosal resistance to ulcerogenic agents in rats. Biol. Pharm. Bull., 18, 223–226 (1995).
Leite, J. P. V., Rastrelli, L., Romussi, G., Oliveira, A. B., Vilegas, J. H. Y., Vilegas, W., and Pizza, C., Isolation and HPLC quantitative analysis of Flavonoid glicosides from Brazilian beverages (Maytenus ilicifolia and Maytenus aquifolium). J. Agric. Food Chem., 49, 3796–3801 (2001).
Lewis, D. A. and Hanson, P. J., Anti-ulcer drugs of plant origin. In: Ellis, G. P., G.B. (Eds.), Progress Medicinal Chemistry, Elsevier Science Publishers, Amsterdam, v. 28, pp. 201–231. (1991).
Morimoto, Y., Shimohara, K., Oshima, S., and Sukamoto, T., Effects of the new anti-ulcer agent KB-5492 on experimental gastric mucosal lesions and gastric mucosal defensive factors, as compared to those of terprenone and cimetidine. Jpn. J. Pharmacol., 57, 595–605 (1991).
Nagy, L., Moszski, G. Y., Tarnok, F., Szalai, M., Poth, I., and Javor, T., Interrelationships between the gastric secretory responses, prostaglandin E2 inhibition and serum level of immunoreactive gastrin in pylorus-ligated and antrectomized rats. Pharmacology, 16, 135–141 (1968).
Niero, R., Moser, R., Busato, A. C. B., Yunes, R. A., Reis, A., and Cechinel-Filho, V., A comparative chemical study of Maytenus ilicifolia Mart. Reiss and Maytenus robusta Reiss (Celastraceae). Z. Naturforsch., 56c, 158–161 (2001).
Niero, R., Mafra, A. P., Lenzi, A. C., Cechinel-Filho, V., Tischer, C. A., Malheiros, A., Souza, M. M., Yunes, R. A., and Monache, F. D., A new triterpene with antinociceptive activity from Maytenus robusta. Nat. Prod. Res., 20, 1315–1320 (2006).
Oliveira, D. M., Silva, G. D. F., Duarte, L. P., and Vieira-Filho, S. A., Chemical constituints isolated from roots of Maytenus acanthophylla Reissek (Celastraceae). Biochem. Syst. Ecol., 34, 661–665 (2006).
Queiroga, C. L., Silva, G. F., Dias, P. C., Possenti, A., and Carvalho, J. E., Evaluation of the antiulcerogenic activity of friedelan-3β-ol and friedelin isolated from Maytenus ilicifolia (Celastraceae). J. Ethnopharmacol., 72, 465–468 (2000).
Schmeda-Hirschmann, G., Astudillo, L., Rodriguez, J. A., Theoduloz, C., and Yáñez, T., Gastroprotective effect of the Mapuche crude drug Araucaria araucana resin and its main constituents. J. Ethnopharmacol., 101, 271–276 (2005).
Shay, H., Komarov, S. A., Fels, S. S., Meranze, D., Gruenstein, M., and Siplet, H., A simple method for the uniform production of gastric ulceration in the rat. Gastroenterology, 5, 43–61 (1945).
Silva, J. L., Silva, R. P., Jorge, R. M., Fátima Silva, G. D., Vieira Filho, S. A., Fonseca, A. P. N. D., and Tagliati, C. A., Avaliação da atividade antiulcerogênica da Maytenus truncata Reiss (Celastraceae). Braz. J. Pharmacog., 15, 30–35 (2005).
Sosa, S., Morelli, C. F., Tubaro, A., Cairoli, P., Speranza, G., and Manitto, P., Anti-inflammatory activity of Maytenus senegalensis root extracts and of maytenoic acid. Phytomedicine, 14, 109–114 (2007).
Szabo, S., Mechanisms of mucosal injury in the stomach and duodenum: time-sequence analysis of morphologic, functional, biochemical and histochemical studies. Scand. J. Gastroenterol., 127, 21–28 (1987).
Tiberti, L. A., Yariwake, J. H., Ndjoko, K., and Hostettmann, K., Identification of flavonols in leaves of Maytenus ilicifolia and M. aquifolium (Celastraceae) by LC/UV/MS analisys. J. Chromatogr. B, 846, 378–384 (2007).
Umamaheswari, M., Asokkumar, K., Rathidevi, R., Sivashanmugam, A. T., Subhadradevi, V., and Ravi, T. K., Antiulcer and in vitro antioxidant activities of Jasminum grandiflorum L. J. Ethnopharmacol., 110, 464–470 (2007).
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de Andrade, S.F., Comunello, E., Noldin, V.F. et al. Antiulcerogenic activity of fractions and 3,15-Dioxo-21α-hydroxy friedelane isolated from Maytenus robusta (Celastraceae). Arch. Pharm. Res. 31, 41–46 (2008). https://doi.org/10.1007/s12272-008-1118-5
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DOI: https://doi.org/10.1007/s12272-008-1118-5