Inhibitory effect of kefiran on ovalbumin-induced lung inflammation in a murine model of asthma
Kefiran is a major component of kefir which is a microbial symbiont mixture that produces jelly-like grains. This study aimed to evaluate the therapeutic availability of kefiran on the ovalbumin-induced asthma mouse model in which airway inflammation and airway hyper-responsiveness were found in the lung. BALB/c mice sensitized and challenged to ovalbumin were treated intra-gastrically with kefiran 1 hour before the ovalbumin challenge. Kefiran significantly suppressed ovalbumin-induced airway hyper-responsiveness (AHR) to inhaled methacholine. Administration of kefiran significantly inhibited the release of both eosinophils and other inflammatory cells into bronchoalveolar lavage (BAL) fluid and lung tissue which was measured by Diff-Quik. Interleukin-4 (IL-4) and interleukin-5 (IL-5) were also reduced to normal levels after administration of kefiran in BAL fluid. Histological studies demonstrate that kefiran substantially inhibited ovalbumin-induced eosinophilia in lung tissue by H&E staining and goblet cell hyperplasia in the airway by PAS staining. Taken above data, kefiran may be useful for the treatment of inflammation of lung tissue and airway hyper-responsiveness in a murine model and may have therapeutic potential for the treatment of allergic bronchial asthma.
Key wordsKefiran Asthma Inflammation Airway hyper-responsiveness Mucus Ovalbumin-induced murine model
broncho-alveolar lavage fluid
hematoxylin and eosin
phosphate buffered saline
intra-peritoneal administration and nebulization
type 2 helper T cell
Unable to display preview. Download preview PDF.
- Farnworth, E. R. and Mainville, I., eds, Kefir: a fermented milk product. Handbook of Fermented Functional Foods. CRC Press, 77–112 (2003).Google Scholar
- Gonzalo, J. A., Lloyd, C. M., Kremer, L., Finger, E., Martinez-A, C., Siegelman, M. H., Cybulsky, M., and Gutierrez-Ramos, J. C., Eosinophil recruitment to the lung in a murine model of allergic inflammation. The role of T cells, chemokines, and adhesion receptors. J. Clin. Invest., 98, 2332–2345 (1996).PubMedCrossRefGoogle Scholar
- Hogan, S. P., Mould, A., Kikutani, H., Ramsay, A. J., and Foster, P. S., Aeroallergen-induced eosinophilic inflammation, lung damage, and airways hyperreactivity in mice can occur independently of IL-4 and allergen-specific immunoglobulins. J. Clin. Invest., 99, 1329–1339 (1997).PubMedCrossRefGoogle Scholar
- La Riviere, J. W. and Kooiman, P., Kefiran, a novel polysaccharide produced in the kefir grain by Lactobacillus brevis. Arch. Microbiol., 59, 269–278 (1967).Google Scholar
- Lee, J. J., McGarry, M. P., Farmer, S. C., Denzler, K. L., Larson, K. A., Carrigan, P. E., Brenneise, I. E., Horton, M. A., Haczku, A., Gelfand, E. W., Leikauf, G. D., and Lee, N. A., Interleukin-5 expression in the lung epithelium of transgenic mice leads to pulmonary changes pathognomonic of asthma. J. Exp. Med., 185, 2143–2156 (1997).PubMedCrossRefGoogle Scholar
- Rankin, J. A., Picarella, D. E., Geba, G. P., Temann, U. A., Prasad, B., DiCosmo, B., Tarallo, A., Stripp, B., Whitsett, J., and Flavell, R. A., Phenotypic and physiologic characterization of transgenic mice expressing interleukin 4 in the lung: lymphocytic and eosinophilic inflammation without airway hyperreactivity. Proc. Natl. Acad. Sci. U S A, 93, 7821–7825 (1996).PubMedCrossRefGoogle Scholar
- Saloff-Coaste, C., Kefir, Danonne Newsletter, 1–11 (1996).Google Scholar