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Neuroprotective effect of benzylideneacetophenone derivative on the MPTP model of neurodegeneration in mice

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Abstract

In this study, we investigated the neuroprotective effect of a benzylideneacetophenone derivative, JC3, in a mouse model of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson’s disease (PD). C57BL/6 mice were treated with MPTP (30 mg/kg, i.p.) for 5 consecutive days. JC3 (10 mg/kg, i.p.) treatment was initiated 2 h after the first administration of MPTP and then at 24-h intervals for 3 consecutive days. The mice were sacrificed for analyses 7 days after the last MPTP injection. Immunohistochemistry and Western blot were used to determine the expression levels of tyrosine hydroxylase (TH), dopamine transporter (DAT), OX-42 (a marker of microglial activation), and glial fibrillary acid protein (GFAP, a marker of astrocyte activation) in the substantia nigra (SN) and striatum (ST). The results of these experiments demonstrated that JC3 restored the decreased TH-immunoreactivity (IR) and DAT and JC3 attenuated the increase in OX-42, GFAP, and COX-2 on the SN and ST on day 7 post-MPTP injection. These results suggest that JC3 can be a neuroprotective agent in an MPTP-induced model of PD.

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Authors and Affiliations

  1. Department of Neuroscience and Medical Research Institute, School of Medicine, Ewha Womans University, Seoul, 158-710, Korea

    Jun Mo Kang, Jae-Chul Jung, Heejeong Kim, Heena Lim, Soyong Jang & Seikwan Oh

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  1. Jun Mo Kang
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  2. Jae-Chul Jung
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  3. Heejeong Kim
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  5. Soyong Jang
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Correspondence to Seikwan Oh.

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Kang, J.M., Jung, JC., Kim, H. et al. Neuroprotective effect of benzylideneacetophenone derivative on the MPTP model of neurodegeneration in mice. Arch. Pharm. Res. 31, 1098–1107 (2008). https://doi.org/10.1007/s12272-001-1275-5

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