Abstract
The SpyTag/SpyCatcher protein ligation technology was applied to create a modular antibody assembly platform. Fab with a SpyTag and a prebuild set of various SpyCatchers were used to generate mono- and bivalent Fab fragments and synthetic IgGs. Biotin, enzyme or dye conjugated SpyCatcher give access to labeled antibodies.
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Ylera, F. Modulare Antikörper durch SpyTag Protein-Ligation erhöht Assayflexibilität. Biospektrum 28, 624–626 (2022). https://doi.org/10.1007/s12268-022-1837-z
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DOI: https://doi.org/10.1007/s12268-022-1837-z