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Automatisierte Analyse der Koloniebildung

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Abstract

The colony formation assay evaluates the proliferative capacity of a single cell. For applications such as cancer drug screening, it is important to distinguish cells that retain this proliferative capacity from those that do not. Conventional analysis of this assay involves scoring and quantifying colonies in each well of a multi-well format manually by eye, limiting its throughput capabilities. We present an automated method for conducting and analyzing the colony formation assay in both a 6-well and 96-well microplate using the upright color brightfield imaging with a wide field of view.

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Literatur

  1. Puck TT, Marcus PI (1955) A rapid method for viable cell titration and clone production with HeLa cells in tissue culture: The use of X-irradiated cells to supply conditioning factors. Proc Natl Acad Sci 41:432–437

    Article  CAS  Google Scholar 

  2. Bufu T, Di X, Yilin Z et al. (2018) Celastrol inhibits colorectal cancer cell proliferation and migration through suppression of MMP3 and MMP7 by the PI3K/AKT signalling pathway. Anticancer Drugs 29:530–538

    Article  CAS  Google Scholar 

  3. Crowley LC, Christensen ME, Waterhouse NJ (2016) Measuring survival of adherent cells with the colony-forming assay. Cold Spring Harb Protoc; doi: https://doi.org/10.1101/pdb.prot087171

  4. Franken NAP, Rodermond HM, Stap J et al. (2006) Clonogenic assay of cells in vitro. Nat Protocols 1:2315–2319

    Article  CAS  Google Scholar 

  5. BioTek Instruments, Inc., https://www.biotek.com/resources/application-notes/high-throughput-fluorescent-colony-formation-assay

  6. Fornari FA, Randolph JK, Yalowich JC et al. (1994) Interference by doxorubicin with DNA unwinding in MCF-7 breast tumor cells. Mol Pharmacol 45:649–656

    CAS  PubMed  Google Scholar 

  7. Pommier Y, Leo E, Zhang HL et al. (2010) DNA topoisomerases and their poisoning by anticancer and antibacterial drugs. Chemistry & Biology 17:421–433

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Biotek Instruments - A Part of Agilent, Winooski, USA

    Ernest Heimsath

  2. BioTek Instruments GmbH - A part of Agilent, Kocherwaldstraße 34, D-74177, Bad Friedrichshall, Deutschland

    Swen Carlstedt

Authors
  1. Ernest Heimsath
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  2. Swen Carlstedt
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Correspondence to Swen Carlstedt.

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Heimsath, E., Carlstedt, S. Automatisierte Analyse der Koloniebildung. Biospektrum 26, 756–757 (2020). https://doi.org/10.1007/s12268-020-1494-z

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  • DOI: https://doi.org/10.1007/s12268-020-1494-z

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