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Gezieltes Entfernen von Fluorochromkonjugaten nach der Zellsortierung

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  • Durchflusszytometrie
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Abstract

Flow cytometric analysis and sorting rely on fluorescent labeling with multiple antibody-fluorochrome conjugates. Currently available conjugates remain on the cells after flow sorting and thus can limit downstream applications. In contrast, REAlease® conjugates enable specific cell labeling and can be completely removed from the epitopes after the experiment; cell epitopes and fluorescence channels that were blocked through labeling become available for subsequent experiments.

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Authors and Affiliations

  1. Miltenyi Biotec B. V. & Co. KG, Friedrich-Ebert-Straße 68, D-51429, Bergisch Gladbach, Deutschland

    Jennifer Pankratz, Sabine Schmachtenberg, Christiane Siewert, Alina Kurowski &  Christian Dose

Authors
  1. Jennifer Pankratz
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  2. Sabine Schmachtenberg
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  3. Christiane Siewert
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  4. Alina Kurowski
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  5. Christian Dose
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Corresponding author

Correspondence to Christian Dose.

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Danksagung

Wir danken Susanne Krauthäuser, Nicole Susann Eckardt, Dmytro A. Yushchenko, Nicole Jansen und Anna Bergmann für ihre Unterstützung.

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Pankratz, J., Schmachtenberg, S., Siewert, C. et al. Gezieltes Entfernen von Fluorochromkonjugaten nach der Zellsortierung. Biospektrum 26, 289–291 (2020). https://doi.org/10.1007/s12268-020-1370-x

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  • DOI: https://doi.org/10.1007/s12268-020-1370-x

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