Abstract
Flow cytometric analysis and sorting rely on fluorescent labeling with multiple antibody-fluorochrome conjugates. Currently available conjugates remain on the cells after flow sorting and thus can limit downstream applications. In contrast, REAlease® conjugates enable specific cell labeling and can be completely removed from the epitopes after the experiment; cell epitopes and fluorescence channels that were blocked through labeling become available for subsequent experiments.
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Wir danken Susanne Krauthäuser, Nicole Susann Eckardt, Dmytro A. Yushchenko, Nicole Jansen und Anna Bergmann für ihre Unterstützung.
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Pankratz, J., Schmachtenberg, S., Siewert, C. et al. Gezieltes Entfernen von Fluorochromkonjugaten nach der Zellsortierung. Biospektrum 26, 289–291 (2020). https://doi.org/10.1007/s12268-020-1370-x
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DOI: https://doi.org/10.1007/s12268-020-1370-x