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High-content imaging zur Identifikation von Replikationsfehlern

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  • DNA-Synthese
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BIOspektrum Aims and scope

Abstract

Dysregulation of DNA-licensing is a hallmark for hereditary diseases, as well as for the development of tumour cells. Here we show how the combination of high-content imaging (HCl) with highly specific antibodies from Proteintech allows deeper insights into the timing of replication licensing following the loading of specific markers onto the DNA. Similar to flow cytometry, HCl can be used to investigate large quantities of cells whilst adding crucial structural information.

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Literatur

  1. Chessel A, Carazo Salas RE (2019) From observing to predicting single-cell structure and function with high-through-put/high-ontent microscopy. Essays Biochem 63:197–208

    Article  Google Scholar 

  2. Thomas N (2010) High-content screening: a decade of evolution. J Biomol Screen 15:1–9

    Article  Google Scholar 

  3. Pegoraro G, Misteli T (2017) High-throughput imaging for the discovery of cellular mechanisms of disease. Trends Genet 33:604–615

    Article  CAS  Google Scholar 

  4. Blow JJ (2019) Defects in the origin licensing checkpoint stresses cells exiting GO. J Cell Biol 218:2080–2081

    Article  CAS  Google Scholar 

  5. Jackson AP, Laskey RA, Coleman N (2014) Replication proteins and human disease. Cold Spring Harb Perspect Biol 6, doi: https://doi.org/10.1101/cshperspect.a013060

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Authors and Affiliations

  1. Proteintech Group, Proteintech Group 4th Floor, 196 Deansgate, UK-Manchester, M3 3WF, UK

    Karolina Szczesna

  2. School of Life Sciences, University of Dundee, Dow Street, UK-Dundee, DD 1 5EH, UK

    Viola Denninger

Authors
  1. Karolina Szczesna
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  2. Viola Denninger
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Corresponding authors

Correspondence to Karolina Szczesna or Viola Denninger.

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Szczesna, K., Denninger, V. High-content imaging zur Identifikation von Replikationsfehlern. Biospektrum 26, 66–68 (2020). https://doi.org/10.1007/s12268-020-1322-5

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  • DOI: https://doi.org/10.1007/s12268-020-1322-5

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