Organisch-chemisch stabilisierte Enzyme für eine nachhaltige Wirtschaft

Abstract

Advances in protein engineering have made the industrial use of enzymes feasible, though, a more wide-spread application is hampered by the limited stability of enzymes under often required harsh conditions. We developed a protein stabilization technology that involves the in situ cyclization of proteins (INCYPRO) and facilitates the design and semi-synthesis of multicyclic enzymes. These synthetically enhanced durable enzymes add to the current efforts towards a sustainable and green economy.

Literatur

  1. [1]

    Li S, Yang X, Yang S et al. (2012) Technology prospecting on enzymes: application, marketing and engineering. Comput Struct Biotechnol J 2, doi: https://doi.org/10.5936/csbj.201209017

  2. [2]

    Gibney E, Van Noorden R, Ledford H et al. (2018) ‘Testtube’ evolution wins Chemistry Nobel Prize. Nature 562:176

    Article  CAS  PubMed  Google Scholar 

  3. [3]

    Bowler BE (2007) Thermodynamics of protein denatured states. Mol BioSyst 3:88

    Article  CAS  PubMed  Google Scholar 

  4. [4]

    Bornscheuer UT, Huisman GW, Kazlauskas RJ et al. (2012) Engineering the third wave of biocatalysis. Nature 485:185–194

    Article  CAS  PubMed  Google Scholar 

  5. [5]

    Pelay-Gimeno M, Bange T, Hennig S et al. (2018) In situ cyclization of native proteins: structure-based design of a bicyclic enzyme. Angew Chem Int Ed 57:11164–11170

    Article  CAS  Google Scholar 

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Correspondence to Tom N. Grossmann.

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Open Access: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, duplication, adaption, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Open access funding provided by Vrije Universiteit Amsterdam, The Netherlands.

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Neubacher, S., Hennig, S. & Grossmann, T.N. Organisch-chemisch stabilisierte Enzyme für eine nachhaltige Wirtschaft. Biospektrum 25, 338–339 (2019). https://doi.org/10.1007/s12268-019-1039-5

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