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GPCR-Aktivierung: Live-Berichterstattung aus der Zelle

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Abstract

Live cell assays with genetically encoded, fluorescent biosensors provide significant advantages over endpoint assays in cell lysates as they acquire the timing of cellular responses in cells relevant to disease. Here we show how second messengers relevant to the Gs/Gi or the Gq signalling pathway are detected in living cells using assays for cAMP, diacylglycerol (DAG), and Ca2+.

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Literatur

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Authors and Affiliations

  1. BMG LABTECH GmbH, Allmendgrün 8, D-77799, Ortenberg, Deutschland

    Andrea Krumm

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  1. Andrea Krumm
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Correspondence to Andrea Krumm.

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Krumm, A. GPCR-Aktivierung: Live-Berichterstattung aus der Zelle. Biospektrum 25, 186–187 (2019). https://doi.org/10.1007/s12268-019-1030-1

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  • DOI: https://doi.org/10.1007/s12268-019-1030-1

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