Abstract
iSCAT microscopy is a powerful tool for the optical detection and visualization of small nanoparticles, down to single unlabeled proteins. In this overview, we give an introduction to the method’s principles and benefits and show how it can be applied to monitor protein secretion of single living cells with single-protein sensitivity at millisecond temporal resolution.
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Katharina König Studium der Angewandten Naturwissenschaft an der TU Bergakademie Freiberg. Seit 2014 Doktorandin bei Prof. Dr. V. Sandoghdar am Max Planck Institut für die Physik des Lichts in Erlangen.
André Gemeinhardt Jahrgang 1990. Physikstudium an der TU Chemnitz und der TU Dresden mit Schwerpunkten nichtlineare Opik und Rasterkraftmikroskopie. Seit 2016 Promotion in der Arbeitsgruppe von Prof. Dr. V. Sandoghdar am Max Planck Institut für die Physik des Lichts in Erlangen auf dem Gebiet der Biophysik und hochauflösenden Mikroskopie.
Vahid Sandoghdar 1987 Bachelor of Science in Physik, University of California, Davis, USA. 1993 Ph.D. in Physik, Yale University, New Haven, USA. Danach Postdoc-Aufenthalt an der Ecole Normale Supérieure in Paris, Frankreich, und Arbeitsgruppenleiter an der Universität Konstanz. 2001 Professor für physikalische Chemie, ETH Zürich, Schweiz. Seit 2011 Direktor am Max-Planck-Institut für die Physik des Lichts in Erlangen und Humboldt-Professor an der Universität Erlangen-Nürnberg. Gründer des Max-Planck-Zentrums für Physik und Medizin, Erlangen.
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König, K., Gemeinhardt, A. & Sandoghdar, V. Interferenz von Licht macht einzelne unmarkierte Proteine sichtbar. Biospektrum 25, 732–736 (2019). https://doi.org/10.1007/s12268-019-0225-9
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DOI: https://doi.org/10.1007/s12268-019-0225-9