Abstract
Bioluminescence emission allows the direct visualization of living cells without external light. Its generation usually requires addition of a luciferin, except for bacterial bioluminescence with the lux operon where synthesis and recycling of the luciferin are performed by enzymatic reactions in the cell. An improved ilux operon produces brighter bioluminescence and enables autonomous bioluminescence imaging with increased sensitivity, allowing the observation of single Escherichia coli cells.
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Carola Gregor 2006–2011 Biochemiestudium an der Universität und Medizinischen Hochschule Hannover. 2011–2016 Promotion am Max-Planck-Institut für biophysikalische Chemie, Göttingen (Doktorgrad verliehen von der Universität Hannover). Seit 2016 Postdoc am Max-Planck-Institut für biophysikalische Chemie, Göttingen.
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Gregor, C. Autonome Lichtproduktion in Bakterien mit optimiertem ilux-Operon. Biospektrum 24, 697–699 (2018). https://doi.org/10.1007/s12268-018-0976-8
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DOI: https://doi.org/10.1007/s12268-018-0976-8