Abstract
Liquid biopsy offers the potential for non-invasive monitoring and mutation detection in cancer. Detection of mutations with very low allele frequencies using PCR based technologies is a technical challenge. Several quantitative PCR based systems are integrated in routine molecular diagnostics. The detection limit may not always suffice with these qPCR systems, but may be achieved by new digital PCR systems.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Diaz-Serrano A, Gella P, Jimenez E et al. (2018) Targeting EGFR in lung cancer: current standards and developments. Drugs, doi: 10.1007/s40265-018-0916-4
Siravegna G, Marsoni S, Siena S et al. (2017) Integrating liquid biopsies into the management of cancer. Nat Rev Clin Oncol 14:531–548
Wan JCM, Massie C, Garcia-Corbacho J et al. (2017) Liquid biopsies come of age: towards implementation of circulating tumour DNA. Nat Rev Cancer 17:223–238
Navarro E, Serrano-Heras G, Castano MJ et al. (2015) Realtime PCR detection chemistry. Clin Chim Acta 439:231–250
Ye S, Dhillon S, Ke X et al. (2001) An efficient procedure for genotyping single nucleotide polymorphisms. Nucleic Acids Res 29:E88–8
Powell MJ, Ganta M, Peletskaya E et al. (2015) High Sensitivity Detection of Tumor Gene Mutations. BAOJ Cancer Sciences 1:001
Syed YY (2016) therascreen® EGFR RGQ PCR Kit: a companion diagnostic for afatinib and gefitinib in non-small cell lung cancer. Mol Diagn Ther 20:191–198
Angulo B, Lopez-Rios F, Gonzalez D (2014) A new generation of companion diagnostics: cobas BRAF, KRAS and EGFR mutation detection tests. Expert Rev Mol Diagn 14:517–524
Leitlinienprogramm Onkologie (Deutsche Krebsgesellschaft, Deutsche Krebshilfe, AWMF) (2018) Prävention, Diagnostik, Therapie und Nachsorge des Lungenkarzinoms, Langversion 1.0, AWMF-Registernummer: 020/007OL, http://leitlinienprogramm-onkologie.de/ Lungen karzinom.98.0.html
Vogelstein B, Kinzler KW (1999) Digital PCR. Proc Natl Acad Sci USA 96:9236–9241
Hindson BJ, Ness KD, Masquelier DA et al. (2011) Highthroughput droplet digital PCR system for absolute quantitation of DNA copy number. Anal Chem 83:8604–8610
Diehl F, Li M, He Y et al. (2006) BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions. Nat Methods 3:551–559
Author information
Authors and Affiliations
Corresponding author
Additional information
Tamara V. Werner 2007–2012 Biologiestudium mit Schwerpunkt Human- und Molekularbiologie an der Universität des Saarlandes, dort 2013–2016 Promotion am Institut für Humangenetik. Seit 2017 Postdoktorandin am Institut für Klinische Pathologie des Universitätsklinikums Freiburg im Rahmen des Deutschen Konsortiums für Translationale Krebsforschung (DKTK).
Silke Laßmann Seit 2002 Forschungsgruppenleiterin am Institut für Klinische Pathologie des Universitätsklinikums Freiburg. Seit 2015 auch naturwissenschaftliche Leitung der molekularpathologischen Routinediagnostik.
Rights and permissions
About this article
Cite this article
Werner, T.V., Laßmann, S. PCR-basierte Verfahren für die Muta - tionsdetektion bei Tumorpatienten. Biospektrum 24, 601–603 (2018). https://doi.org/10.1007/s12268-018-0964-z
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12268-018-0964-z