Konformationsbewegungen von aktiven Membrantransportern


Mechanistic understanding of biological and biochemical processes requires methods to analyze structures and states but also conformational dynamics of biomacromolecules at room temperature under physiologically relevant conditions. Single-molecule Förster resonance energy transfer (smFRET) has evolved to a versatile tool for exactly this, the observation of intra- and intermolecular conformational dynamics and interactions of biomacromolecules. We here outline the basic principles and illustrate the applications of smFRET for understanding molecular mechanisms of active membrane transporters.


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Correspondence to Thorben Cordes.

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Rebecca Mächtel Jahrgang 1991. Biologiestudium an der Universität Erlangen-Nürnberg (B. Sc.) und TU München (M. Sc.), mit Aufenthalt an der Fondazione Edmund Mach, San Michele all‘Adige, Italien. Seit 2017 Promotion in Biologie an der LMU München.

Christian Gebhardt Jahrgang 1992. Physik- und Informatikstudium an der LMU München, mit Aufenthalt am Centre de Recherche Paul Pascal, Bordeaux, Frankreich. 2017 wissenschaftlicher Mitarbeiter am Max-Planck-Institut für Quantenoptik, Garching. Seit 2017 Promotion in Biologie an der LMU München.

Thorben Cordes Jahrgang 1980. Chemiestudium an der TU Braunschweig, mit Aufenthalt am University College Cork, Irland. 2008 Promotion in Physik an der LMU München, 2008–2009 Postdoc dort und 2010–2011 an der Universität Oxford, UK. 2011–2016 Assistant Professor, 2016–2017 Associate Professor an der Universität Groningen, Niederlande. Seit 2017 Professor für Physikalische und Synthetische Biologie an der LMU München.

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Mächtel, R., Gebhardt, C. & Cordes, T. Konformationsbewegungen von aktiven Membrantransportern. Biospektrum 24, 495–497 (2018). https://doi.org/10.1007/s12268-018-0945-2

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