BIOspektrum

, Volume 24, Issue 3, pp 274–277 | Cite as

Nano- und Chromobodies für high-content und super-resolution imaging

  • Bettina-Maria Keller
  • Julia Maier
  • Sören Segan
  • Björn Tränkle
  • Ulrich Rothbauer
Wissenschaft · Special: High Content Imaging Lebendzellmikroskopie
  • 4 Downloads

Abstract

Nano- and chromobodies are recombinant binding molecules that have expanded the possibilities of advanced cellular imaging. Here we provide an overview how chromobodies can be implemented to visualize localization and dynamics of endogenous proteins in live-cell high-content imaging. Additionally, we highlight recent advances in the development of nanobodies to label cellular structures with high densities and minimal fluorophore displacement for super-resolution microscopy.

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Copyright information

© Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature 2018

Authors and Affiliations

  • Bettina-Maria Keller
    • 1
  • Julia Maier
    • 2
  • Sören Segan
    • 2
  • Björn Tränkle
    • 1
  • Ulrich Rothbauer
    • 1
    • 2
  1. 1.Pharmazeutische BiotechnologieUniversität TübingenTübingenDeutschland
  2. 2.Naturwissenschaftliches und Medizinisches InstitutUniversität Tübingen Pharmazeutische BiotechnologieReutlingenDeutschland

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