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BIOspektrum

, Volume 24, Issue 3, pp 278–282 | Cite as

Protein-Cross-Linking zur Aufklärung von komplexen Strukturen

  • Olexandr Dybkov
  • Alexandra Stützer
  • Karl Bertram
  • Berthold Kastner
  • Holger Stark
  • Reinhard Lührmann
  • Henning Urlaub
Open Access
Wissenschaft · Special: High Content Imaging Kryo-Elektronenmikroskopie
  • 66 Downloads

Abstract

Cryo-electron microscopy (cryo-EM) can solve structures of highly dynamic macromolecular complexes. To characterize less well defined regions in cryo-EM images, cross-linking coupled with mass spectrometry (CX-MS) provides valuable information on the arrangement of domains and amino acids. CX-MS involves covalent linkage of protein residues close to each other and identifying these connections by mass spectrometry. Here, we summarise the advances of CX-MS and its integration with cryo-EM for structural reconstruction.

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Copyright information

© Die Autoren 2018

Open Access:

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, duplication, adaption, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Open access funding provided by Max Planck Society.

Authors and Affiliations

  • Olexandr Dybkov
    • 1
  • Alexandra Stützer
    • 2
  • Karl Bertram
    • 3
  • Berthold Kastner
    • 1
  • Holger Stark
    • 3
  • Reinhard Lührmann
    • 1
  • Henning Urlaub
    • 2
    • 4
  1. 1.Abteilung Zelluläre BiochemieMax-Planck-Institut für Biophysikalische ChemieGöttingenDeutschland
  2. 2.Bioanalytische MassenspektrometrieMax-Planck-Institut für biophysikalische ChemieGöttingenDeutschland
  3. 3.Abteilung Strukturelle DynamikMax-Planck-Institut für Biophysikalische ChemieGöttingenDeutschland
  4. 4.Bioanalytik, Institut für Klinische ChemieUniversitätsmedizin GöttingenGöttingenDeutschland

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