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BIOspektrum

, Volume 24, Issue 1, pp 43–45 | Cite as

Automatisierte Quantifizierung proteinöser Partikel

  • Oliver Bannach
  • Andreas Kulawik
  • Christian Zafiu
  • Dieter Willbold
Wissenschaft · Special: Liquid Handling & Probenvorbereitung sFIDA-Technologie
  • 20 Downloads

Abstract

sFIDA is a platform technology for specific and sensitive quantitation of single proteinaceous particles, such as amyloid-β oligomers as an early biomarker for Alzheimer’s disease (AD). Automation improves robustness and performance of the sFIDA technology, which is a prerequisite for high-throughput measurements and future application of the technology in routine AD diagnostics and other applications.

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Literatur

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    Wang-Dietrich L, Funke SA, Kühbach K et al. (2013) The amyloid-beta oligomer count in cerebrospinal fluid is a biomarker for Alzheimer’s disease. J Alzheimers Dis 34:985–994PubMedGoogle Scholar
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    Bannach O, Kühbach K, Willbold D (2015) Surface-based fluorescence intensity distribution analysis (sFIDA): Ein neues Verfahren zur Frühdiagnose der Alzheimerschen Demenz. BIOspektrum 6:728–730CrossRefGoogle Scholar
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    Hülsemann M, Zafiu C, Kühbach K et al. (2016) Biofunctionalized silica nanoparticles: standards in amyloid-β oligomer-based diagnosis of Alzheimer’s disease. J Alzheimers Dis 54:79–88CrossRefPubMedGoogle Scholar
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    Herrmann Y, Kulawik A, Kühbach K et al. (2017) sFIDA automation yields subfemtomolar limit of detection for Aβ aggregates in body fluids. Clin Biochem 50:244–247CrossRefPubMedGoogle Scholar
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    Herrmann Y, Bujnicki T, Zafiu C et al. (2017) Nanoparticle standards for immuno-based quantitation of α-synuclein oligomers in diagnostics of Parkinson’s disease and other synucleinopathies. Clin Chim Acta 466:152–159CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature 2018

Authors and Affiliations

  • Oliver Bannach
    • 1
    • 2
  • Andreas Kulawik
    • 1
    • 2
  • Christian Zafiu
    • 2
  • Dieter Willbold
    • 1
    • 2
  1. 1.Institut für Physikalische BiologieHHU DüsseldorfDüsseldorfDeutschland
  2. 2.Institute of Complex Systems Structural Biochemistry (ICS-6)Forschungszentrum Jülich GmbHJülichDeutschland

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