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BIOspektrum

, Volume 24, Issue 1, pp 34–36 | Cite as

Identifizierung von Substratbindestellen in der γ-Sekretase

  • Akio Fukumori
  • Johannes Trambauer
  • Lukas P. Feilen
  • Harald Steiner
Wissenschaft · Methoden Photoaffinitätsmarkierung
  • 20 Downloads

Abstract

The lack of detailed information on the interactions between substrates and enzymes is a major gap in understanding the mechanisms of many proteases. Using the Alzheimer’s disease protease γ-secretase as an example, we show how a previously developed versatile chemical biology approach allows to systematically identify molecular interactions with the enzyme at the amino acid level of the substrate. Furthermore, this approach provides additional mechanistic insights that could also aid in the development of advanced drugs against this key protease.

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Copyright information

© Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature 2018

Authors and Affiliations

  • Akio Fukumori
    • 1
  • Johannes Trambauer
    • 2
  • Lukas P. Feilen
    • 3
  • Harald Steiner
    • 2
    • 3
  1. 1.Department of Aging NeurobiologyNational Center for Geriatrics and Gerontology und Osaka University Graduate School of MedicineOsakaJapan
  2. 2.Biomedizinisches Centrum (BMC) Lehrstuhl für StoffwechselbiochemieLudwig-Maximilians-Universität München Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)MünchenDeutschland
  3. 3.Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)MünchenDeutschland

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