Skip to main content
Log in

Baukasten der Natur: neue Proteine aus konservierten Fragmenten

  • Wissenschaft
  • Proteindesign
  • Published:
BIOspektrum Aims and scope


Proteins evolved to be very diverse and adapted to a multitude of central cellular functions. This impressive repertoire of today’s proteins developed through duplication and recombination of smaller protein and peptide building blocks. Based on such evolutionary observations we proposed a rational design strategy in which new functional hybrids can be build from fragments of existing proteins. With this approach we are tackling the long-standing goal of complex custom-made protein design.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others


  1. Söding J, Lupas AN (2003) More than the sum of their parts: on the evolution of proteins from peptides. Bioessays 25:837–846

    Article  PubMed  Google Scholar 

  2. Söding J (2005) Protein homology detection by HMM-HMM comparison. Bioinformatics 21:951–960

    Article  PubMed  Google Scholar 

  3. Farias-Rico JA, Schmidt S, Höcker B (2014) Evolutionary relationship of two ancient protein superfolds. Nat Chem Biol 10:710–715

    Article  CAS  PubMed  Google Scholar 

  4. Höcker B, Schmidt S, Sterner R (2002) A common evolutionary origin of two elementary enzyme folds. FEBS Lett 510:133–135

    Article  PubMed  Google Scholar 

  5. Höcker B (2014) Design of proteins from smaller fragments–learning from evolution. Curr Opin Struct Biol 27:56–62

    Article  PubMed  Google Scholar 

  6. Bharat TA, Eisenbeis S, Zeth K et al. (2008) A beta alpha-barrel build by the combination of fragments from different folds. Proc Natl Acad Sci USA 105:9942–9947

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Eisenbeis S, Proffitt W, Coles M et al. (2012) Potential of fragment recombination for rational design of proteins. J Am Chem Soc 134:4019–4022

    Article  CAS  PubMed  Google Scholar 

  8. Shanmugaratnam S, Eisenbeis S, Höcker B (2012) A highly stable protein chimera built from fragments of different folds. Protein Eng Des Sel 25:699–703

    Article  CAS  PubMed  Google Scholar 

  9. Alva V, Söding J, Lupas AN (2015) A vocabulary of ancient peptides at the origin of folded proteins. Elife 4:e09410

    Article  PubMed  PubMed Central  Google Scholar 

  10. Farias-Rico JA, Höcker B (2013). Design of chimeric proteins by combination of subdomain-sized fragments. Methods Enzymol 523:389–405

    Article  Google Scholar 

  11. Huang PS, Feldmeier K, Parmeggiani F et al. (2016) De novo design of a fourfold symmetric TIM-barrel protein with atomic level accuracy. Nat Chem Biol 12:29–34

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Birte Höcker.

Additional information

Saacnicteh Toledo-Patino Biochemie- und Pharmaziestudium an der Michoacana de San Nicolas de Hidalgo Universität Morelia, Mexico, sowie Biochemie an der Universität Tübingen. 2013 Diplomarbeit am Max-Planck-Institut für Entwicklungsbiologie, Tübingen, dort seit 2014 Doktorandin in der AG „Protein-design“ und seit 2016 an der Universität Bayreuth.

Francisco Lobos 2011 Bachelor in Bioengineering an der University of Concepcion, Chile, dort 2014 Master in Biochemie und Bioinformatik. Seit 2015 Doktorand der International Max Planck Research School (IMPRS) Tübingen in der AG „Proteindesign“ am Max-Planck-Institut für Entwicklungsbiologie, Tübingen, und seit 2016 an der Universität Bayreuth.

Birte Höcker Biologiestudium an den Universitäten Göttingen und Ottawa, Kanada. 2003 Promotion im Fach Biochemie an der Universität Köln. 20032005 Postdoc am Duke Univer sity Medical Center, Durham, NC, USA. 20062016 Gruppenleiterin am Max-Planck-Institut für Entwicklungsbiologie, Tübingen. Seit 2016 W3-Professorin für Biochemie an der Universität Bayreuth. 2015 ERC Consolidator Award und Otto-Meyerhof-Preis der GBM.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Toledo-Patino, S., Lobos, F. & Höcker, B. Baukasten der Natur: neue Proteine aus konservierten Fragmenten. Biospektrum 23, 630–633 (2017).

Download citation

  • Published:

  • Issue Date:

  • DOI: