Abstract
Perfusion becomes a reasonable alternative to traditional batch or fed-batch processes, partly due to the increasing availability of suitable re-usable or single-use equipment. High cell densities and long duration of perfusion processes require reliable process control. Amongst others, monitoring of cell stress becomes important. Intact cell MALDI-TOF mass spectrometry (ICM-MS) biotyping is described here as a robust at-line monitoring method of cell stress in perfusion cell culture.
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Sebastian Schwamb Jahrgang 1984. Biotechnologiestudium (FH) in Bingen. 2008–2011 Research Scientist bei Ganymed Pharmaceuticals AG, Mainz. 2011–2015 Promotion an der Universität Heidelberg in Kooperation mit der Hochschule Mannheim. Seit 2015 Laborleiter Upstream Development bei Sanofi Aventis Deutschland GmbH.
Philipp Wiedemann Jahrgang 1968. Maschinenbaustudium in Clausthal-Zellerfeld, Biologiestudium an der Universität Bonn. 2000 Promotion. 2001–2003 Research Scientist bei Nucleics Pty Ltd., Sydney, Australien. 2003–2006 Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach, zuletzt stellvertretender Betriebsleiter Fermentation. Seit 2007 Professur für Pharmazeutische Biotechnologie an der Hochschule Mannheim.
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Schwamb, S., Wiedemann, P. At-line Monitoring von Zellkulturprozessen mittels Massenspektrometrie. Biospektrum 23, 399–402 (2017). https://doi.org/10.1007/s12268-017-0817-1
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DOI: https://doi.org/10.1007/s12268-017-0817-1