Skip to main content
SpringerLink
Log in

Aufklärung toxischer Wirkmechanismen mittels Hochdurchsatz-RT-qPCR

Genexpressionsanalysen

  • Anwendungen & Produkte
  • Published:
BIOspektrum Aims and scope

Abstract

Gene expression studies have become valuable tools to identify the complex mode of action of toxic or carcinogenic substances. In our group we established a quantitative high-throughput RT-qPCR technique using Fluidigm dynamic arrays on the BioMark™ System to analyze the expression level of 95 genes in 96 samples in parallel. By creating a special gene set comprising critical genes of the DNA damage response system the impact on genomic stability can be investigated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. Fischer B, Neumann D, Piberger A et al. (2015) Use of high-throughput RT-qPCR to assess modulations of gene expression profiles related to genomic stability and inter - actions by cadmium. Arch Toxicol, doi: 10.1007/s00204-015-1621-7

    Google Scholar 

  2. Harper JW, Elledge SJ (2007) The DNA damage response: ten years after. Mol Cell 28:739–745

    Article  CAS  PubMed  Google Scholar 

  3. Zhou BB, Elledge SJ (2000) The DNA damage response: putting checkpoints in perspective. Nature 408:433–439

    Article  CAS  PubMed  Google Scholar 

  4. Spurgeon SL, Jones RC, Ramakrishnan R (2008) High throughput gene expression measurement with real time PCR in a microfluidic dynamic array. PLoS One 3:e1662

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Abteilung Lebensmittelchemie und Toxikologie, Institut für Angewandte Biowissenschaften (IAB), Karlsruher Institut Für Technologie (KIT), Adenauerring 20a, D-76131, Karlsruhe, Deutschland

    Bettina Maria Fischer & Andrea Hartwig

Authors
  1. Bettina Maria Fischer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Andrea Hartwig
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Andrea Hartwig.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fischer, B.M., Hartwig, A. Aufklärung toxischer Wirkmechanismen mittels Hochdurchsatz-RT-qPCR. Biospektrum 22, 499–500 (2016). https://doi.org/10.1007/s12268-016-0718-8

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12268-016-0718-8

Search

Navigation