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Analyse des Mikromilieus künstlicher Gewebe mit Protein-Multiplex-Assays

Stammzelldifferenzierung

  • Wissenschaft · Special: Laborautomation
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Abstract

The development of bioartificial tissues using stem cells has an enormous potential in medicine. To determine effects of differentiation strategies on cell micro-milieus, a fast and efficient method is necessary. The protein multiplex assay is based on enzyme-linked immunosorbent assays and allows automated, quantitative and qualitative analysis of multiple proteins in small sample volumes. Here, this method is shown to verify differentiation of mesenchymal stem cells into endothelial cells.

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Author information

Authors and Affiliations

  1. Institut für Technische Chemie, Universität Hannover, Callinstraße 5, D-30167, Hannover, Deutschland

    Stefanie Thoms, Rebecca Jonczyk, Antonina Lavrentieva & Cornelia Blume

Authors
  1. Stefanie Thoms
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  2. Rebecca Jonczyk
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  3. Antonina Lavrentieva
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  4. Cornelia Blume
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Corresponding author

Correspondence to Cornelia Blume.

Additional information

Stefanie Thoms 2009–2014 Life-Science-Studium, Universität Hannover. Seit 2015 Doktorandin am Institut für Technische Chemie, Universität Hannover.

Antonina Lavrentieva 1993–1998 Biologiestudium, Staatliche Lomonosow Universität, Moskau, Russland. 1998–2004 Promotion am Institut für Physiologie, Russische Akademie der Medizinischen Wissenschaft, Moskau. 2008–2012 Promotion und seit 2013 Postdoktorandin am Institut für Technische Chemie, Universität Hannover.

Rebecca Jonczyk 2004–2010 Chemiestudium (Diplom), Universität Hannover. 2010–2014 Promotion und seit 2014 Postdoktorandin am Institut für Technische Chemie, Universität Hannover.

Cornelia Blume 1987–1994 Medizinstudium, Ruhr-Universität Bochum. 2005–2009 Oberärztin Nephrologie, Universität Düsseldorf, Habilitation. 2009–2013 Oberärztin Nephrologie/Hypertensiologie, Medizinische Hochschule Hannover, dort 2014 apl. Professur. Seit 2013 Gruppenleiterin am Institut für Technische Chemie, Universität Hannover, im Exzellenzcluster „Biofabrication for NIFE“ (Land Niedersachsen).

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Thoms, S., Jonczyk, R., Lavrentieva, A. et al. Analyse des Mikromilieus künstlicher Gewebe mit Protein-Multiplex-Assays. Biospektrum 22, 471–474 (2016). https://doi.org/10.1007/s12268-016-0714-z

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  • DOI: https://doi.org/10.1007/s12268-016-0714-z

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