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Protein-Nukleinsäure-Interaktionen im Massenspektrometer

Strukturbiologie

  • Wissenschaft · Methoden
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BIOspektrum Aims and scope

Abstract

Protein-nucleic acid complexes play a significant role in all DNA- and RNA-related cellular processes. The identification of protein-nucleic acid interaction sites is important to understand the structure, function and regulation of these complexes. Here, new approaches in mass spectrometry using UV-induced cross-linking of nucleic acids with proteins have recently proven as an important technique to not only identify proteins but specific regions of proteins that interact with RNA or DNA.

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Authors and Affiliations

  1. Bioanalytische Massenspektrometrie, Max-Planck-Institut für Biophysikalische Chemie, Am Fassberg 11, Göttingen, D-37077, Deutschland

    Alexandra Stützer, Aleksandar Chernev & Henning Urlaub

  2. Institut Für Klinische Chemie, Universitätsmedizin, Universität Göttingen, Göttingen, Deutschland

    Aleksandar Chernev & Henning Urlaub

Authors
  1. Alexandra Stützer
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  2. Aleksandar Chernev
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  3. Henning Urlaub
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Corresponding author

Correspondence to Henning Urlaub.

Additional information

Alexandra Stützer 2002–2007 Biochemiestudium an der Universität Leipzig. 2007–2011 Promotion am Max-Planck-Institut für Biophysikalische Chemie, Göttingen. 2011–2015 Postdoktorandin in der AG Fischle. Seit 2015 Postdoktorandin in der AG Urlaub.

Aleksandar Chernev 2008–2012 Molekularbiologiestudium (Bachelor) an der Universität Sofia, Bulgarien. Seit 2012 MSc/PhD Molecular Biology Program (IMPRS) der Universität Göttingen und seit 2014 Doktorand in der AG Urlaub am Max-Planck-Institut für Biophysikalische Chemie, Göttingen.

Henning Urlaub Biochemiestudium an der FU Berlin, Doktorarbeit am Max-Delbrück-Centrum in Berlin (Buch) und Promotion an der FU Berlin. Seit 2005 unabhängiger Forschungsgruppenleiter (Bioanalytische Massenspektrometrie) am Max-Planck-Institut für Biophysikalische Chemie, Göttingen. Seit 2010 Professor in der Universitätsmedizin Göttingen und Leiter der Forschungsgruppe Bioanalytik am Institut für Klinische Chemie.

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Stützer, A., Chernev, A. & Urlaub, H. Protein-Nukleinsäure-Interaktionen im Massenspektrometer. Biospektrum 22, 466–469 (2016). https://doi.org/10.1007/s12268-016-0713-0

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  • DOI: https://doi.org/10.1007/s12268-016-0713-0

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