BIOspektrum

, Volume 22, Issue 4, pp 395–397 | Cite as

3D-Migrationsassays im Hochdurchsatz

  • Brad Larson
  • Leonie Rieger
  • Hubert Tseng
  • Glauco R. Souza
  • Aleksandra Velkova-Krei
3D-Zellkultur
  • 30 Downloads

Abstract

Migration assays are a common tool to screen toxic effects of compounds. However, assays using monolayer cell cultures may not entirely represent a native in vivo environment and may result in misinterpretation of compound toxicity. Magnetic 3D bioprinting in combination with automated kinetic imaging provides an easy and robust high-throughput screening approach of compound effects on cell migration in a 3D environment.

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Literatur

  1. [1]
    Werner S, Krieg T, Smola H (2007) Keratinocyte-fibroblast interactions in wound healing. J Invest Dermatol 127:998–1008CrossRefPubMedGoogle Scholar
  2. [2]
    Souza GR, Molina JR, Raphael RM et al. (2010) Threedimensional tissue culture based on magnetic cell levitation. Nat Nanotechnol 5:291–296CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Brad Larson
    • 1
  • Leonie Rieger
    • 1
  • Hubert Tseng
    • 2
  • Glauco R. Souza
    • 2
  • Aleksandra Velkova-Krei
    • 3
  1. 1.Biotek InstrumentsWinooskiUSA
  2. 2.Nano3D BiosciencesHoustonUSA
  3. 3.Greiner Bio-One GmbHFrickenhausenGermany

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