Abstract
Genetic engineering of metabolic pathways for biotechnological purposes is often hampered by factors like slow diffusion rates, competing pathways or secretion of intermediates. To bypass these limitations, compartimentalization of enzymatic reactions is a promising approach. For this, enzymes can either be arranged in complexes or they can be isolated in coated subcellular compartments. We are developing new concepts to assemble various enzymes in protein supercomplexes and to relocate metabolic pathways into synthetic organelles.
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Mara Reifenrath, Joanna Tripp, Mislav Oreb und Eckhard Boles (v. l. n. r.) arbeiten am Institut für Molekulare Biowissenschaften der Universität Frankfurt a. M. an neuartigen Konzepten zur Kompartimentierung biotechnologisch interessanter Stoffwechselwege in Hefen.
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Reifenrath, M., Tripp, J., Oreb, M. et al. Synthetische subzelluläre Kompar - timente in eukaryotischen Zellen. Biospektrum 22, 374–377 (2016). https://doi.org/10.1007/s12268-016-0701-4
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DOI: https://doi.org/10.1007/s12268-016-0701-4