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BIOspektrum

, Volume 22, Issue 4, pp 365–367 | Cite as

Reversibel schaltbare fluoreszierende Proteine für die Superauflösung

  • Martin Andresen
  • Nickels Jensen
  • Stefan Jakobs
Nanoskopie
  • 64 Downloads

Abstract

Super-resolution microscopy, or nanoscopy, enables the visualization of cellular structures inaccessible to conventional light microscopy. RESOLFT nanoscopy is especially suitable for the imaging of living cells. It requires fluorescent proteins (RSFPs) that can be repeatedly switched between a fluorescent and a non-fluorescent state by light. We have analyzed the molecular switching mechanisms and generated a family of RSFPs specifically tailored for live cell RESOLFT nanoscopy.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Martin Andresen
    • 1
  • Nickels Jensen
    • 1
  • Stefan Jakobs
    • 1
  1. 1.Abteilung NanoBiophotonikMax-Planck-Institut für biophysikalische ChemieGottingenGermany

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