Reversibel schaltbare fluoreszierende Proteine für die Superauflösung
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Super-resolution microscopy, or nanoscopy, enables the visualization of cellular structures inaccessible to conventional light microscopy. RESOLFT nanoscopy is especially suitable for the imaging of living cells. It requires fluorescent proteins (RSFPs) that can be repeatedly switched between a fluorescent and a non-fluorescent state by light. We have analyzed the molecular switching mechanisms and generated a family of RSFPs specifically tailored for live cell RESOLFT nanoscopy.
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- Grotjohann T, Testa I, Reuss M et al. (2012) rsEGFP2 enables fast RESOLFT nanoscopy of living cells. eLife 1:e00248Google Scholar